Hormonal regulation of protein degradation in hepatoma cells in tissue culture.
Hormonal regulation of protein degradation in hepatoma cells in tissue culture.
复制标题
组织培养中肝癌细胞蛋白质降解的激素调节。
DOI：
10.1210/endo-94-3-676
复制
发表时间：
1974
期刊：
影响因子：
4.8
通讯作者：
J. Emanuel
中科院分区：
文献类型：
--
作者：
T. Gelehrter;J. Emanuel
地塞米松和胰岛素对细胞总蛋白降解的影响已在建立的大鼠肝癌细胞系（HTC）悬浮培养中通过测量酸溶性放射性从先前标记的蛋白质的释放进行了研究。当在化学成分确定的无血清培养基中孵育时，HTC细胞每小时损失约2%至3%的标记蛋白。地塞米松（0.1 μM）导致蛋白质降解表观速率增加20%。向地塞米松处理的细胞中加入胰岛素（0.1 U/ml），通过一种不需要伴随RNA合成的机制使蛋白质降解速率降低26%。由于放线菌素D阻断了胰岛素刺激的蛋白质中氨基酸掺入的适度增强，但不影响胰岛素对蛋白质分解的抑制，因此胰岛素对蛋白质降解的影响似乎与其对蛋白质合成的影响无关。牛血清（5%），它模拟了胰岛素的稳定性。
The effects of dexamethasone and insulin on the degradation of total cellular protein have been investigated in an established line of rat hepatoma cells (HTC) in suspension culture by measuring the release of acid-soluble radioactivity from previously labeled protein. When incubated in a chemically-defined, serum-free medium, HTC cells lose approximately 2 to 3% of their labeled protein per hour. Dexamethasone (0.1 μM) causes a 20% increase in the apparent rate of protein degradation. The addition of insulin (0.1 U/ml) to dexamethasone-treated cells causes a 26% decrease in the rate of protein degradation by a mechanism which does not require concomitant RNA synthesis. Since actinomycin D blocks the modest enhancement of amino acid incorporation into protein stimulated by insulin, but does not affect the inhibition of protein breakdown by insulin, it appears that the effect of insulin on protein degradation is independent of its effects on protein synthesis. Bovine serum (5%), which mimics the insulin st...