High-speed Atomic Force Microscopy for Capturing Dynamic Behavior of Protein Molecules at Work
High-speed Atomic Force Microscopy for Capturing Dynamic Behavior of Protein Molecules at Work
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DOI：
10.1380/ejssnt.2005.384
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发表时间：
2005-01-01
影响因子：
0.7
通讯作者：
Matada, Keiko
中科院分区：
文献类型：
--
作者：
Ando, Toshio;Kodera, Noriyuki;Matada, Keiko
似乎只有原子力显微镜 (AFM) 才有可能以高空间和时间分辨率追踪活动中的蛋白质。为了实现这一潜力，我们一直在开发高速 AFM。我们不仅努力提高扫描速度，而且还尽可能减少尖端与样品的相互作用力。高速AFM可以在视频中捕获移动的蛋白质分子和弱相互作用的蛋白质分子，而不干扰其生理功能。在这里，我们回顾了过去 10 年进行的研究，以及我们为下一代仪器所做的前期工作。
It seems that only the atomic force microscope (AFM) has a potential to trace a protein in action with high spatial and temporal resolution. To realize that potential, we have been developing a high-speed AFM. We have made efforts not only to enhance the scan speed but also to reduce the tip-sample interaction force as much as possible. The high-speed AFM can capture moving protein molecules and weakly interacting protein molecules on video without disturbing their physiological function. Here we review our studies carried out over the past 10 years, and our preliminary work toward the next generation of the instrument.