Rumen microbial abundance and fermentation profile during severe subacute ruminal acidosis and its modulation by plant derived alkaloids in vitro
Rumen microbial abundance and fermentation profile during severe subacute ruminal acidosis and its modulation by plant derived alkaloids in vitro
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DOI：
10.1016/j.anaerobe.2016.02.002
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发表时间：
2016-06-01
期刊：
影响因子：
2.3
通讯作者：
Zebeli, Qendrim
中科院分区：
文献类型：
--
作者：
Mickdam, Elsayed;Khiaosa-ard, Ratchaneewan;Zebeli, Qendrim
瘤胃微生物群对宿主动物具有重要的代谢功能。本研究的目的是表征瘤胃微生物丰度和发酵概况的变化，使用严重的亚急性瘤胃酸中毒（SARA）在体外模型，并评估植物衍生的生物碱（PDA），含有季苯并菲啶和原阿片碱生物碱，其中血根碱和白屈菜红碱的主要生物活性化合物的潜在调节作用。严重SARA的诱导强烈影响瘤胃微生物组成和发酵变量，而不抑制细菌总数的丰度。原虫和真菌对瘤胃低pH的敏感性高于细菌。重度SARA的诱导明显抑制了纤维的降解（P < 0.001），这与纤维溶解性白色瘤胃球菌和产琥珀酸纤维杆菌的相对丰度下降（P < 0.001）是沿着的。在严重的SARA条件下，普雷沃氏菌属、乳杆菌属、埃氏巨球腔菌和内甲藻属。（P < 0.001），而嗜淀粉反刍动物的丰度较低。SARA在很大程度上抑制了甲烷形成（-70%，P < 0.001），尽管总的产甲烷16 S rRNA基因丰度不受影响。主成分分析表明，甲烷短杆菌属（Methanobrevibacter spp.）与甲烷浓度相关。在正常条件下，添加PDA可显著提高瘤胃内总SCFA、丙酸和戊酸的浓度（P < 0.05），并显著提高粗蛋白质的降解率（P < 0.05）。我们的研究结果表明，与正常条件相比，严重SARA期间微生物群落发生了强烈变化。在正常瘤胃pH条件下，PDA的补充积极调节瘤胃发酵。(C)2016爱思唯尔有限公司版权所有
Rumen microbiota have important metabolic functions for the host animal. This study aimed at characterizing changes in rumen microbial abundances and fermentation profiles using a severe subacute ruminal acidosis (SARA) in vitro model, and to evaluate a potential modulatory role of plant derived alkaloids (PDA), containing quaternary benzophenanthridine and protopine alkaloids, of which sanguinarine and chelerythrine were the major bioactive compounds. Induction of severe SARA strongly affected the rumen microbial composition and fermentation variables without suppressing the abundance of total bacteria. Protozoa and fungi were more sensitive to the low ruminal pH condition than bacteria. Induction of severe SARA clearly depressed degradation of fiber (P < 0.001), which came along with a decreased relative abundance of fibrolytic Ruminococcus albus and Fibrobacter succinogenes (P < 0.001). Under severe SARA conditions, the genus Prevotella, Lactobacillus group, Megasphaera elsdenii, and Entodinium spp. (P < 0.001) were more abundant, whereas Ruminobacter amylophilus was less abundant. SARA largely suppressed methane formation (-70%, P < 0.001), although total methanogenic 16S rRNA gene abundance was not affected. According to principal component analysis, Methanobrevibacter spp. correlated to methane concentration. Addition of PDA modulated ruminal fermentation under normal conditions such as enhanced (P < 0.05) concentration of total SCFA, propionate and valerate, and increased (P < 0.05) degradation of crude protein compared with the unsupplemented control diet. Our results indicate strong shifts in the microbial community during severe SARA compared to normal conditions. Supplementation of PDA positively modulates ruminal fermentation under normal ruminal pH conditions. (C) 2016 Elsevier Ltd. All rights reserved.