Effect of Oral Infection of Mayaro Virus on Fitness Correlates and Expression of Immune Related Genes inAedes aegypti
Effect of Oral Infection of Mayaro Virus on Fitness Correlates and Expression of Immune Related Genes inAedes aegypti
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DOI：
10.3390/v12070719
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发表时间：
2020-07-01
期刊：
影响因子：
4.7
通讯作者：
Shin, Dongyoung
中科院分区：
文献类型：
--
作者：
Alto, Barry W.;Civana, Ayse;Shin, Dongyoung
马亚罗病毒是一种蚊媒甲病毒，在南美洲热带森林中流行，有一个涉及非人灵长类动物和嗜血蚊的森林循环。人类感染马亚罗病毒会导致发热性疾病和长期关节痛，病例通常与暴露于热带森林栖息地有关。热带森林栖息地和城市环境之间的人员流动可能允许输入病例和随后通过国内蚊子埃及伊蚊的本地传播。的相对重要性。埃及伊蚊作为马亚罗病毒载体的致病性可能取决于病毒对适合度的影响，特别是那些与载体能力有关的昆虫学参数。我们进行了蚊子感染的研究，并比较了成年生存和繁殖力的女性从巴西和佛罗里达州人口的Ae。埃及伊蚊口服摄入非感染性（对照）和马亚罗病毒感染性血液后。马亚罗病毒感染和难治性蚊子的繁殖力与对照（未暴露）蚊子相似或低30-50%，这表明建立免疫应答或难治性表型表达的生殖成本。马亚罗病毒感染并没有改变成年雌蚊的存活率以及Toll和IMD通路中靶向基因的表达。成虫寿命和繁殖力估计是独立的蚊子体内测得的病毒滴度。感染状态对雌性存活率无不良影响，表明马亚罗病毒不会改变该参数变化介导的媒介能力。
Mayaro virus is a mosquito-borneAlphavirusendemic to forests of tropical South America with a sylvatic cycle involving non-human primates andHaemagogusmosquitoes. Human infection with Mayaro virus causes a febrile illness and long-lasting arthralgia and cases are often associated with exposure to tropical forest habitats. Human movement between tropical forest habitats and urban settings may allow for imported cases and subsequent local transmission by domestic mosquitoAedes aegypti. The relative importance ofAe. aegyptias a vector of Mayaro virus may depend on the pathogenic effects of the virus on fitness correlates, especially those entomological parameters that relate to vectorial capacity. We performed mosquito infection studies and compared adult survival and fecundity of females from Brazilian and Floridian populations ofAe. aegyptifollowing oral ingestion of uninfectious (control) and Mayaro virus infectious blood. Mayaro virus infected and refractory mosquitoes had similar or 30-50% lower fecundity than control (unexposed) mosquitoes, suggesting a reproductive cost to mounting an immune response or phenotypic expression of refractoriness. Survival of adult female mosquitoes and targeted gene expression in the Toll and IMD pathways were not altered by Mayaro virus infection. Adult lifespan and fecundity estimates were independent of measured viral titer in the bodies of mosquitoes. The lack of adverse effects of infection status on female survival suggests that Mayaro virus will not alter vectorial capacity mediated by changes in this parameter.