LECTINS ARE SENSITIVE TOOLS FOR DEFINING THE DIFFERENTIATION PROGRAMS OF MOUSE GUT EPITHELIAL-CELL LINEAGES
LECTINS ARE SENSITIVE TOOLS FOR DEFINING THE DIFFERENTIATION PROGRAMS OF MOUSE GUT EPITHELIAL-CELL LINEAGES
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DOI：
10.1152/ajpgi.1994.266.6.g987
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发表时间：
1994-06-01
影响因子：
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通讯作者：
GORDON, JI
中科院分区：
文献类型：
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作者：
FALK, P;ROTH, KA;GORDON, JI
我们已经使用组织化学方法来调查的细胞模式的结合的一组45凝集素与明确定义的碳水化合物的特异性，从胎儿，新生儿和成年FVB/N小鼠肠道的胃-结肠轴的各个区域制备的部分。结果表明，凝集素可以被用来作为非常敏感的工具来描述的分化程序的胃和肠上皮细胞系作为其位置的函数沿着的头尾轴的肠道和作为一个功能的发展阶段。研究表明，许多这些细胞系特异性和空间模式的糖缀合物的生产可以建立和维持在没有暴露于管腔内容物和存在特定的增殖异常的情况下，肠同种移植和转基因小鼠，表达猴病毒-40 T抗原的肠上皮细胞。这个凝集素面板应是有用的操作定义亚群的主要肠道上皮细胞谱系在正常品系的小鼠，描述在突变和转基因小鼠的肠道上皮细胞分化程序的变化，并恢复特定的上皮细胞谱系或亚群。
We have used histochemical methods to survey the cellular patterns of binding of a panel of 45 lectins with well-defined carbohydrate specificities to sections prepared from various regions of the gastric-to-colonic axis of fetal, neonatal, and adult FVB/N mouse gut. The results suggest that lectins can be used as remarkably sensitive tools to describe the differentiation programs of gastric and intestinal epithelial cell lineages as a function of their position along the cephalocaudal axis of the gut and as a function of developmental stage. Studies of intestinal isografts and transgenic mice that express Simian virus-40 T antigen in enterocytes suggest that many of these cell lineage-specific and spatial patterns of glycoconjugate production can be established and maintained in the absence of exposure to luminal contents and in the presence of specific proliferative abnormalities. This lectin panel should be useful for operationally defining subpopulations of the principal gut epithelial cell lineages in normal strains of mice, for describing variations in gut epithelial cell differentiation programs in mutant and transgenic mice, and for recovering specific epithelial cell lineages or subpopulations.