Correlation between mitochondrial TRAP-1 expression and lymph node metastasis in colorectal cancer
Correlation between mitochondrial TRAP-1 expression and lymph node metastasis in colorectal cancer
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DOI：
10.3748/wjg.v18.i41.5965
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发表时间：
2012-11-07
影响因子：
4.3
通讯作者：
Lu, Yuan-Ming
中科院分区：
文献类型：
--
作者：
Gao, Jing-Yan;Song, Bao-Rong;Lu, Yuan-Ming
目的：探讨线粒体肿瘤坏死因子受体相关蛋白-1(TRAP-1)在结直肠癌淋巴转移(LNM)中的作用，并利用实时定量聚合酶链式反应(RT-PCR)技术，寻找潜在的LNM相关生物标志物。方法：应用定量RT-PCR、Western blotting和免疫组织化学方法检测96例结直肠癌组织中线粒体TRAP-1基因表达的差异。结果：RT-PCR、Western blotting和免疫组织化学方法证实，与非LNM型结直肠癌相比，LNM型结直肠癌组织中TRAP-1表达显著上调。用Western blotting分析TRAP-1在两种不同转移潜能的人结直肠癌细胞株LoVo和HT29中的表达。LoVo中TRAP-1的表达水平显著高于HT29。TRAP-1的过表达与淋巴结转移(90.2%vs22%，P<；0.001)、晚期肿瘤转移分期(89.1%vs26.9%，P<；0.001)、5年复发率(82.7%vs22.6%，P<；)显著相关；5年总生存率下降(48.4%vs83.2%，P<；0.001)。单因素和多因素分析显示，TRAP-1表达是影响结直肠癌患者复发和生存的独立预后因素(复发风险比为2.445，P=0.017；生存风险比为2.867，P=0.028)。结论：线粒体TRAP-1影响结直肠癌的淋巴结转移，可能成为判断结直肠癌淋巴结转移的潜在生物学指标和预后因素。TRAP-1的过度表达是结直肠癌患者预后不良的预测因素。(C)2012年白石登。版权所有。
AIM: To evaluate the effect of mitochondrial tumor necrosis factor receptor-associated protein-1 (TRAP-1) on the lymph node metastasis (LNM) in Chinese colorectal cancer (CRC) patients, and develop potential LNM-associated biomarkers for CRC using quantitative real-time polymerase chain reaction (RT-PCR) analysis.METHODS: Differences in mitochondrial TRAP-1 gene expression between primary CRC with LNM (LNM CRC) and without LNM (non-LNM CRC) were assessed in 96 Chinese colorectal carcinoma samples using quantitative RT-PCR analysis, Western blotting, and confirmed with imnnunohistochemical assay. The relationship between clinicopathological parameters and potential diagnostic biomarkers was also examined.RESULTS: TRAP-1 was significantly upregulated in LNM CRC compared with non-LNM CRC, which was confirmed by RT-PCR, Western blotting and immunohistochemical assay. The expression of TRAP-1 in two different metastatic potential human colorectal cancer cell lines, LoVo and HT29, was analyzed with Western blotting. The expression level of TRAP-1 was dramatically higher in LoVo than in HT29. Overexpression of TRAP-1 was significantly associated with LNM (90.2% in LNM group vs 22% in non-LNM group, P < 0.001), the advanced tumor node metastasis stage (89.1% in LNM group vs 26.9% in non-LNM group, P < 0.001), the increased 5-year recurrence rate (82.7% in LNM group vs 22.6% in non-LNM group, P < 0.001) and the decreased 5-year overall survival rate (48.4% in LNM vs 83.2% in non-LNM group, P < 0.001). Univariate and multivariate analyses indicated that TRAP-1 expression was an independent prognostic factor for recurrence and survival of CRC patients (Hazard ratio of 2.445 in recurrence, P = 0.017; 2.867 in survival, P = 0.028).CONCLUSION: Mitochondria TRAP-1 affects the lymph node metastasis in CRC, and may be a potential bio-marker for LNM and a prognostic factor in CRC. Overexpression of TRAP-1 is a predictive factor for the poor outcome of colorectal cancer patients. (C) 2012 Baishideng. All rights reserved.