Lack of involvement of μ1 opioid receptors in dermorphin-induced inhibition of hypoxic and hypercapnic ventilation in rat pups
Lack of involvement of μ1 opioid receptors in dermorphin-induced inhibition of hypoxic and hypercapnic ventilation in rat pups
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DOI：
10.1016/s1569-9048(02)00030-7
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发表时间：
2002-08-01
影响因子：
2.3
通讯作者：
Miller, JH
中科院分区：
文献类型：
--
作者：
Colman, AS;Miller, JH
在2-21日龄清醒、无拘束的Wistar大鼠中研究了皮吗啡（一种p-选择性阿片受体激动剂）对出生后发育过程中对改变的O和CO的呼吸反应的影响。通过气压体积描记法记录呼吸。皮吗啡（4 mg kg（-1））皮下给药，（11% O-2，89% N-2）在2-21日龄幼仔和高氧（100%，O-2）和高碳酸血症（8%CO2，92%O-2）在存在和不存在β 1受体拮抗剂纳洛嗪（10 mg kg（-1）s.c.）在测试前一天给药。6分钟的缺氧增加了所有年龄组的通气量，主要是通过增加频率。皮吗啡可抑制低氧诱导的呼吸反应，但这种抑制作用对纳洛嗪不敏感。高氧5分钟后，通气与空气呼吸相同，除了在存在dermorphin的情况下，当高氧通气被纳洛嗪不敏感的频率降低抑制时。在此5分钟100%O-2暴露后，将幼仔暴露于高碳酸血症，并在5分钟后测量呼吸参数。皮吗啡以纳洛嗪不敏感的方式抑制对CO2的兴奋反应。在任何年龄的大鼠幼仔中，没有证据表明内源性μ受体调节对改变的气体的呼吸反应。因此，μ阿片诱导的抑制年轻大鼠的缺氧和高碳酸血症的反应不会发生通过激活μ阿片受体。(C)2002 Elsevier Science B. V.保留所有权利。
The effects of dermorphin, a p-selective opioid agonist, on respiratory responses to altered 0, and CO, during postnatal development were investigated in conscious, unrestrained Wistar rats aged 2-21 days. Respiration was recorded by barometric plethysmography. Dermorphin (4 mg kg(-1)) was administered subcutaneously, and the ventilatory responses to hypoxia (11% O-2, 89% N-2) in 2-21-day-old pups and hyperoxia (100%, O-2), and hypercapnia (8% CO2, 92% O-2) in 2-13-day-old pups were assessed in the presence and absence of the p, receptor antagonist naloxonazine (10 mg kg(-1) s.c.) administered I day before testing. Six minutes of hypoxia increased ventilation in all age groups, largely via an increase in frequency. Dermorphin inhibited the ventilatory response to hypoxia, and this inhibition was insensitive to naloxonazine. After 5 min of hyperoxia, ventilation was the same as with air breathing except in the presence of dermorphin, when hyperoxic ventilation was depressed by a naloxonazinc-inscnsitive decrease in frequency. Following this 5 min 100% O-2 exposure, pups were exposed to hypercapnia, and respiratory parameters were measured 5 min later. The ventilatory response to CO2 was inhibited by dermorphin in a naloxonazine-insensitive manner. There was no evidence for endogenous mu receptor modulation of the ventilatory responses to altered gases in rat pups of any age. Thus, mu opioid-induced inhibition of the hypoxic and hypercapnic responses in young rats does not occur via activation of mu opioid receptors. (C) 2002 Elsevier Science B.V. All rights reserved.