Effects of receptor for advanced glycation endproducts on microvessel formation in endometrial cancer.
Effects of receptor for advanced glycation endproducts on microvessel formation in endometrial cancer.
复制标题
晚期糖基化终末产物受体对子宫内膜癌微血管形成的影响。
DOI：
10.1186/s12885-016-2126-3
复制
发表时间：
2016-02-12
期刊：
影响因子：
3.8
通讯作者：
Ma XX
中科院分区：
文献类型：
--
作者：
Zheng L;Li D;Zhou YM;Yang H;Cheng D;Ma XX
晚期糖基化终末产物受体（receptor for advanced glycation endproducts，RECEPTOR）和微血管状态在癌症进展中均起关键作用。然而，子宫内膜癌中微血管形成和肿瘤之间的相互影响在很大程度上仍然未知。检测20例正常子宫内膜组织、37例高分化子宫内膜癌组织和35例低分化子宫内膜癌组织中VEGF的表达和微血管密度。采用回归分析法分析血管密度与血管密度的关系。在HEC-1A子宫内膜癌细胞中，使用小干扰RNA实现了对E2的敲低。异种移植肿瘤模型用于评估RAGE介导的微血管形成和子宫内膜癌细胞的增殖。研究表明：（i）RAGE表达分别在正常子宫内膜、高分化子宫内膜癌和低分化子宫内膜癌中逐渐增加;（ii）人子宫内膜癌样本中RAGE与微血管密度存在正相关性;（iii）RAGE敲低可有效减少异种移植肿瘤模型中的微血管形成;和（iv）在体内，β 2受体的敲低可以显著抑制子宫内膜癌细胞的增殖。这些结果表明，在子宫内膜癌的发展过程中，子宫内膜异位症可能是微血管形成和增殖的潜在触发因素。本文的在线版本（doi：10.1186/s12885-016-2126-3）包含补充材料，可供授权用户使用。
The receptor for advanced glycation endproducts (RAGE) and microvascular status both play a critical role in cancer progression. However, the crosstalk between RAGE and microvascular formation in endometrial cancer remains largely unknown. RAGE expression and microvessel density were examined in 20 cases of normal endometrial tissue, 37 cases of well-differentiated endometrial cancer tissue, and 35 cases of poorly-differentiated endometrial cancer tissue. Regression analysis was used to examine the relationship between RAGE and microvessel density. The knockdown of RAGE was achieved using a small interfering RNA in HEC-1A endometrial cancer cells. A xenografted tumour model was used to evaluate RAGE-mediated microvascular formation and proliferation of endometrial cancer cells. It was shown that (i) RAGE expression gradually increased in normal endometrium, well-differentiated endometrial cancer, and poorly-differentiated endometrial cancer, respectively; (ii) a positive correlation existed between RAGE and microvessel density in human endometrial cancer samples; (iii) RAGE knockdown was effective in decreasing microvessel formation in xenografted tumour models; and (iv) RAGE knockdown can significantly inhibit the proliferation of endometrial cancer cells in vivo. These results indicate that RAGE may be a potential trigger in microvascular formation and proliferation in the development of endometrial cancer. The online version of this article (doi:10.1186/s12885-016-2126-3) contains supplementary material, which is available to authorized users.