CD10 expression identifies a subset of human perivascular progenitor cells with high proliferation and calcification potentials
CD10 expression identifies a subset of human perivascular progenitor cells with high proliferation and calcification potentials
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CD10 表达鉴定出具有高增殖和钙化潜力的人类血管周围祖细胞亚群
DOI：
10.1002/stem.3112
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发表时间：
2019-11-19
期刊：
影响因子：
5.2
通讯作者：
Peault, Bruno
中科院分区：
文献类型：
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作者：
Ding, Lijun;Vezzani, Bianca;Peault, Bruno
外膜包裹着动脉和静脉，并含有参与血管重塑的假定间充质干细胞（MSC）。我们在这里展示了人类外膜细胞的一个子集表达 CD10/CALLA 细胞表面金属蛋白酶。 CD10+ 和 CD10− 外膜细胞在培养中扩增时均表现出 MSC 的表型特征。然而，与 CD10- 外膜细胞相比，CD10+ 外膜细胞表现出更高的增殖、克隆形成和成骨潜力。 CD10+外膜细胞通过 ERK1/2 信号传导增加细胞周期蛋白 CCND2 的表达，并通过 NF-κB 信号传导增加成骨细胞基因的表达。通过音刺猬介导的 GLI1 信号传导，外膜细胞中 CD10 的表达上调。这些结果表明，CD10 在其他正常和恶性细胞谱系中标记快速分裂的细胞，在血管周围 MSC 功能和细胞命运规范中发挥作用。这些发现还指出了 CD10+ 血管周围细胞在血管重塑和钙化中的作用。
The tunica adventitia ensheathes arteries and veins and contains presumptive mesenchymal stem cells (MSCs) involved in vascular remodeling. We show here that a subset of human adventitial cells express the CD10/CALLA cell surface metalloprotease. Both CD10+ and CD10− adventitial cells displayed phenotypic features of MSCs when expanded in culture. However, CD10+ adventitial cells exhibited higher proliferation, clonogenic and osteogenic potentials in comparison to their CD10− counterparts. CD10+ adventitial cells increased expression of the cell cycle protein CCND2 via ERK1/2 signaling and osteoblastogenic gene expression via NF‐κB signaling. CD10 expression was upregulated in adventitial cells through sonic hedgehog‐mediated GLI1 signaling. These results suggest that CD10, which marks rapidly dividing cells in other normal and malignant cell lineages, plays a role in perivascular MSC function and cell fate specification. These findings also point to a role for CD10+ perivascular cells in vascular remodeling and calcification.