Silkrose: A novel acidic polysaccharide from the silkmoth that can stimulate the innate immune response.
Silkrose: A novel acidic polysaccharide from the silkmoth that can stimulate the innate immune response.
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DOI：
10.1016/j.carbpol.2015.09.070
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发表时间：
2016-01
影响因子：
11.2
通讯作者：
Takashi Ohta;Kie Kusano;A. Ido;C. Miura;T. Miura
中科院分区：
文献类型：
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作者：
Takashi Ohta;Kie Kusano;A. Ido;C. Miura;T. Miura
我们已经确定了一种新的酸性多糖从蚕蛾（天蚕）pueridum激活哺乳动物的先天免疫反应。使用小鼠RAW264巨噬细胞中的一氧化氮产生作为免疫刺激活性的指标来分离这种生物活性多糖。我们将这种多糖命名为“丝玫瑰”。它的分子量为3.15 × 105，由9个单糖组成。表达谱表明，丝石竹诱导存在于MyD88依赖性和MyD88非依赖性信号通路下游的促炎细胞因子和干扰素β的表达。此外，抑制Toll样受体4（TLR4），它存在的信号通路的上游，导致抑制NO的产生由丝。此外，该多糖促进RAW264细胞中核因子κ B的活化，表明其通过TLR 4信号通路刺激巨噬细胞中各种细胞因子的诱导。因此，我们的研究结果表明，丝绸激活先天免疫反应的各种病原微生物和病毒感染。
We have identified a novel acidic polysaccharide from silkmoth (Antheraea yamamai) pupae that activates the mammalian innate immune response. This bioactive polysaccharide was isolated using nitric oxide production in mouse RAW264 macrophages as an indicator of immunostimulatory activity. We named this polysaccharide “silkrose”. It has a molecular weight of 3.15 × 105and comprises nine monosaccharides. The expression profiles indicated that silkrose induced the expression of proinflammatory cytokines and interferon β that exist downstream of MyD88-dependent and MyD88-indeptendent signaling pathways. Also, the inhibition of Toll-like receptor 4 (TLR4), which exists upstream of the signaling pathways, led to the suppression of NO production by silkrose. Furthermore, this polysaccharide promoted the activation of nuclear factor kappa B in RAW264 cells, indicating that it stimulates the induction of various cytokines in macrophages through the TLR4 signaling pathway. Our results thus suggest that silkrose activates the innate immune response to various pathogenic microorganisms and viral infections.