Isolation and characterization of polymorphic DNA microsatellite markers from Schistosoma japonicum
Isolation and characterization of polymorphic DNA microsatellite markers from Schistosoma japonicum
复制标题
DOI：
10.1046/j.1471-8286.2003.00466.x
复制
发表时间：
2003-09-01
期刊：
影响因子：
--
通讯作者：
Webster, JP
中科院分区：
文献类型：
--
作者：
Shrivastava, J;Barker, GC;Webster, JP
证明了微卫星位点揭示日本血吸虫遗传多样性的能力。分离出11个新的微卫星标记，评估80 S内的变异性。来自三个中国和一个菲律宾种群的日本血吸虫个体。新的引物也在S. mansoni和S.埃及血吸虫8个引物对所有的S. japonicum群，三个扩增的S. mansoni DNA，但没有扩增出S.嗜血杆菌DNA在27株分离到的沙门氏菌中，只有6株被检出。曼氏特异性引物扩增S.大豆DNA等位基因多样性和观察到的杂合性范围分别为4至21和0.05至0.82，表明高变异性。
The ability of microsatellite loci to reveal genetic diversity within the trematode Schistosoma japonicum is demonstrated. Eleven novel microsatellite markers were isolated, assessing variability within 80 S. japonicum individuals from three Chinese and one Philippine population. Novel primers were also tested upon S. mansoni and S. haematobium. Eight primers showed polymorphic amplification from all S. japonicum groups, three amplified S. mansoni DNA, but none amplified S. haematobium DNA. Only six of 27 previously isolated S. mansoni-specific primers amplified S. japonicum DNA. Allelic diversity and observed heterozygosity ranged from 4 to 21 and 0.05 to 0.82, respectively, suggesting high variability.