RADIOIMMUNOASSAY OF FIBRINOPEPTIDE-A - IMMUNOREACTIVITY OF FIBRINOGEN AND FIBRINOGEN FRAGMENTS
RADIOIMMUNOASSAY OF FIBRINOPEPTIDE-A - IMMUNOREACTIVITY OF FIBRINOGEN AND FIBRINOGEN FRAGMENTS
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DOI：
10.1016/0049-3848(80)90035-3
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发表时间：
1980-01-01
影响因子：
7.5
通讯作者：
KOCKUM, C
中科院分区：
文献类型：
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作者：
KOCKUM, C
当人纤维蛋白肽A（FPA）是纤维蛋白原或纤维蛋白原片段的一部分时，抗纤维蛋白肽A抗血清对FPA具有不同的免疫化学特异性，用于FPA的放射免疫测定，以证明天然分子和纯化分子对降解的不同敏感性。未处理的人血浆纤维蛋白原与1种抗血清K2具有FPA免疫反应性，相当于纤维蛋白原中FPA计算总量的0.02%，而不同批次的纯化纤维蛋白原交叉反应相当于纤维蛋白原中FPA计算总量的0.4-8.7%。不同批次的交叉反应性与其可透析FPA免疫反应性相关，并随着储存和孵育时间的延长而增加，含FPA纤维蛋白原片段的交叉反应性也是如此。
Anti-fibrinopeptide A antisera with different immunochemical specificities for human fibrinopeptide A (FPA), when it is part of fibrinogen or fibrinogen fragments, were used in the radioimmunoassay of FPA to demonstrate different susceptibility of native and purified molecules to degradation. Unprocessed human plasma fibrinogen had a FPA immunoreactivity with 1 antiserum, K2, equivalent to 0.02% of the calculated total amount of FPA in the fibrinogen, while different batches of purified fibrinogen cross-reacted equivalent to 0.4-8.7% of their calculated total content of FPA in the fibrinogen. The cross-reactivity of the different batches was correlated with their dialyzable FPA immunoreactivities and was increased with storage and longer incubation times, as was the cross-reactivity of FPA-containing fibrinogen fragments.