Evaluation of Poly(glycidyl methacrylate)-Coated Column for Enantioseparation with Azithromycin Lactobionate and Clindamycin Phosphate as Chiral Selectors in Capillary Electrophoresis
Evaluation of Poly(glycidyl methacrylate)-Coated Column for Enantioseparation with Azithromycin Lactobionate and Clindamycin Phosphate as Chiral Selectors in Capillary Electrophoresis
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DOI：
10.1007/s10337-021-04029-8
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发表时间：
2021-03
期刊：
影响因子：
1.7
通讯作者：
Xiaodong Sun;Wen Ding;Cheng Chen;Tao Yu;Qin Chen;Ke-Wu Yang
中科院分区：
文献类型：
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作者：
Xiaodong Sun;Wen Ding;Cheng Chen;Tao Yu;Qin Chen;Ke-Wu Yang
本研究以两种抗生素为选择剂，构建了聚甲基丙烯酸甘油酯纳米颗粒（PGMA NP）包被的对映体分离柱体系。通过简单的开环反应可以很容易地引入毛细管柱内的PGMA NP涂层。以5种基本药物为模型，评价了以乳酸阿奇霉素（AL）和磷酸克林霉素（CP）为基础的分离体系的对映选择性。系统地研究了手性选择剂浓度、背景电解质pH、外加电压和系统温度等因素对手性分离分辨率的影响。在优化的条件下，所有对映体的分离都得到了改善，模型药物的柱效率最高可达120,000 plates/m。运行间、逐日和柱间EOF的相对标准偏差重复性小于4.32%。此外，利用AutoDock进行分子建模，阐明AL/CP识别外消旋药物的潜在机制。图形抽象
In this study, a poly(glycidyl methacrylate) nanoparticle (PGMA NP)-coated column system with two antibiotics as selector was constructed for enantioseparation. The PGMA NP coating inside the capillary columns could be easily introduced by a simple ring open reaction. Five basic drugs were used as the model to evaluate the enantioselectivity of Azithromycin Lactobionate (AL) and Clindamycin phosphate (CP)-based separation system. Factors that influence the chiral separation resolution were systematically investigated, such as chiral selector concentration, background electrolyte (BGE) pH, applied voltage and system temperature. Under the optimized conditions, improved enantioseparations were obtained for all enantiomers and the maximum column efficiency of model drugs could reach 120,000 plates/m. The repeatability of relative standard deviations for EOF representing run-to-run, day-to-day and column-to-column was less than 4.32%. In addition, molecular modeling with AutoDock was applied to elucidate the explored potential mechanism of AL/CP for recognizing racemic drugs.Graphic Abstract