Identification of QTLs controlling cold tolerance in cucumber (Cucumis sativus L.) seedlings
Identification of QTLs controlling cold tolerance in cucumber (Cucumis sativus L.) seedlings
复制标题
控制黄瓜幼苗耐寒性QTL的鉴定
DOI：
10.1016/j.scienta.2022.111383
复制
发表时间：
2022-08-01
影响因子：
4.3
通讯作者：
Gu, Xingfang
中科院分区：
文献类型：
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作者：
Li, Caixia;Dong, Shaoyun;Gu, Xingfang
黄瓜（Cucumis sativus L.）是一种重要的经济蔬菜作物，对冷胁迫非常敏感。黄瓜幼苗在极端低温胁迫下会严重降低黄瓜产量，但目前尚未鉴定出耐寒基因。以抗寒自交系CG104和抗寒自交系CG37为材料，构建了186个重组自交系（RILs）群体，对黄瓜幼苗抗寒相关基因位点进行了鉴定。对RILs群体冷害指数（CII）的遗传分析表明，黄瓜幼苗的抗寒性是定量遗传的。重复检测到一个qCT6.1 QTL。由两个侧翼标记（6SSR13741和6SSR19970）划分，在两个不同的实验中分别解释了6.2%和11.0%的表型变异。此外，使用1500个F-2个体，包含41个注释基因，将qCT6.1划分到271.99 kb的区域（两侧标记Indel8和Indel9）。通过序列和表达分析，我们推测编码AP2/ b3结构域的CsaV3 6G042810可能是调控黄瓜耐冷性的候选基因。这些结果为深入了解黄瓜耐冷性的遗传基础，加强分子育种奠定了坚实的基础。
Cucumber (Cucumis sativus L.), an essential and economically important vegetable crop, is sensitive to cold stress. Cucumber seedlings exposed to extreme cold stress seriously reduce cucumber yield, however, few cold-tolerant genes have been identified yet. In this study, a 186 recombinant inbred lines (RILs) population constructed by crossing two inbred lines, cold-tolerant `CG104' and cold-sensitive 'CG37', was used to identify loci associated with cold tolerance in cucumber seedlings. Genetic analysis of the chilling injury index (CII) of the RILs population showed that cold tolerance in cucumber seedlings was quantitatively inherited. One QTL named qCT6.1 was repeatedly detected. It was delimited by two flanking markers (6SSR13741 and 6SSR19970) and explained 6.2% and 11.0% of the phenotypic variation in two different experiments, respectively. Furthermore, the qCT6.1 was delimited to a 271.99 kb region (flanking markers Indel8 and Indel9) using 1500 F-2 individuals, containing 41 annotated genes. Via sequence and expression analysis, we speculated that CsaV3 6G042810, encoding AP2/B3-domain, was likely to be the candidate gene regulating cucumber cold tolerance. These results provide insight into the genetic basis of cucumber cold tolerance and lay a solid basis for enhancing molecular breeding.