Development of imaging techniques to study the pathogenesis of biosafety level 2/3 infectious agents.
Development of imaging techniques to study the pathogenesis of biosafety level 2/3 infectious agents.
复制标题
开发成像技术来研究生物安全 2/3 级传染原的发病机制。
DOI：
10.1111/2049-632x.12199
复制
发表时间：
2014
影响因子：
3.3
通讯作者：
Eugenin,EliseoA
中科院分区：
文献类型：
--
作者：
Rella,CourtneyE;Ruel,Nancy;Eugenin,EliseoA
尽管微生物学和分子生物学在过去几十年中取得了重大进展，但几种传染病仍然是全球关注的问题，每年导致全球数百万人死亡。根据疾病控制中心(CDC)2012年的数据，全球有3400万人感染艾滋病毒，870万新增结核病病例，5亿肝炎病例，5000-1亿人感染登革热。其中一些病原体虽然发病率很高，但没有可靠的临床检测方法。已经产生了新的或改进的方案，以使用高端显微镜(光学显微镜、共焦显微镜和风暴显微镜)和成像软件来增强对几种病原体的检测和定量。在目前的手稿中，我们讨论了这些方法以及这些方法背后的理论。因此，成像技术的进步将为发现治疗干预措施以减少或消除传染病的破坏性后果打开新的可能性。
Despite significant advances in microbiology and molecular biology over the last decades, several infectious diseases remain global concerns, resulting in the death of millions of people worldwide each year. According to the Center for Disease Control (CDC) in 2012, there were 34 million people infected with HIV, 8.7 million new cases of tuberculosis, 500 million cases of hepatitis, and 50–100 million people infected with dengue. Several of these pathogens, despite high incidence, do not have reliable clinical detection methods. New or improved protocols have been generated to enhance detection and quantitation of several pathogens using high-end microscopy (light, confocal, and STORM microscopy) and imaging software. In the current manuscript, we discuss these approaches and the theories behind these methodologies. Thus, advances in imaging techniques will open new possibilities to discover therapeutic interventions to reduce or eliminate the devastating consequences of infectious diseases.