CASTE AND METAMORPHOSIS - HEMOLYMPH TITERS OF JUVENILE-HORMONE AND ECDYSTEROIDS IN LAST INSTAR HONEYBEE LARVAE
CASTE AND METAMORPHOSIS - HEMOLYMPH TITERS OF JUVENILE-HORMONE AND ECDYSTEROIDS IN LAST INSTAR HONEYBEE LARVAE
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DOI：
10.1016/0016-6480(90)90085-z
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发表时间：
1990-07-01
影响因子：
2.7
通讯作者：
HARTFELDER, K
中科院分区：
文献类型：
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作者：
RACHINSKY, A;STRAMBI, C;HARTFELDER, K
保幼激素（JH）和蜕皮激素滴度是影响意大利蜜蜂末龄幼虫等级发育和变态的关键因素。两种高灵敏度的放射免疫测定法用于测定血淋巴中的这些激素。对于保幼激素，这是最重要的控制种姓的发展，蜜蜂，我们的数据显示，种姓特定的高峰在女王幼虫的早期五龄。第二个高峰出现在两个等级的包皮，这可能是负责蛹蜕皮的调节。在两种种姓的包皮中均观察到蜕皮甾类的单峰。然而，在蜂王中，滴度的增加明显早于工蜂幼虫。通过高效液相色谱分离，然后用放射免疫测定法测定此prepupal峰的蜕皮激素组合物。Makisterone A被证明是主要的蜕皮甾类化合物，但20-hydroxyecdysone也被发现在显着量。
Juvenile hormone (JH) and ecdysteroid titers are critical factors for caste development and metamorphosis in the last larval instar of the honeybee, Apis mellifera. Two highly sensitive radioimmunoassays were used for the determination of these hormones in the hemolymph. For juvenile hormone, which is of prime importance for the control caste development in honeybees, our data showed a caste-specific peak in queen larvae of the early fifth instar. A second peak appears in prepupae of both castes which probably is responsible for the regulation of the pupal moult. A single peak of ecdysteroids was observed in prepupae of both castes. In queens, however, the titer increases distinctly earlier than in worker larvae. The ecdysteroid composition of this prepupal peak was determined by high-performance liquid chromatography separation followed by radioimmunoassay. Makisterone A proved to be the main ecdysteroid compound, but 20-hydroxyecdysone was also found in significant amounts.