Gene expression profiling of Drosophila tracheal fusion cells
Gene expression profiling of Drosophila tracheal fusion cells
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DOI：
10.1016/j.gep.2014.05.004
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发表时间：
2014-07-01
影响因子：
1.2
通讯作者：
Jiang, Lan
中科院分区：
文献类型：
--
作者：
Chandran, Rachana R.;Iordanou, Ekaterini;Jiang, Lan
果蝇气管是研究管状器官形成基本机制的首要遗传系统。气管融合细胞引导分支融合过程形成相互连接的管状网络。因此，果蝇气管内的融合细胞将成为研究哺乳动物肾、血管等管状器官分支融合的良好模型。融合过程是一个动态的细胞过程，包括细胞迁移、粘附、囊泡运输、细胞骨架重排和膜融合。为了了解这些细胞事件是如何协调的，我们开始了组装融合细胞的基因表达谱的关键步骤。在这项研究中，我们分析了融合细胞发育过程中234个潜在的气管表达基因在融合细胞中的表达。共发现143个气管基因编码转录因子、信号蛋白、细胞骨架和基质蛋白、转运蛋白和功能未知的蛋白。这些基因根据其在融合细胞中的表达水平与原位杂交显示的邻近非融合细胞中的表达水平被分为四个亚组：(1)在融合细胞中具有相对高丰度的基因，(2)在融合细胞中动态表达的基因，(3)在融合细胞中具有相对低丰度的基因，以及(4)在融合细胞和非融合气管细胞中表达水平相似的基因。本研究确定了融合细胞的表达谱，并假设提出了融合过程所必需的基因，这些基因在融合的不同阶段发挥作用，如表达的位置和时间所示。这些数据将为全面了解分支融合的分子和细胞机制提供基础。(C) 2014 Elsevier B.V.版权所有
The Drosophila trachea is a premier genetic system to investigate the fundamental mechanisms of tubular organ formation. Tracheal fusion cells lead the branch fusion process to form an interconnected tubular network. Therefore, fusion cells in the Drosophila trachea will be an excellent model to study branch fusion in mammalian tubular organs, such as kidneys and blood vessels. The fusion process is a dynamic cellular process involving cell migration, adhesion, vesicle trafficking, cytoskeleton rearrangement, and membrane fusion. To understand how these cellular events are coordinated, we initiated the critical step to assemble a gene expression profile of fusion cells. For this study, we analyzed the expression of 234 potential tracheal-expressed genes in fusion cells during fusion cell development. 143 Tracheal genes were found to encode transcription factors, signal proteins, cytoskeleton and matrix proteins, transporters, and proteins with unknown function. These genes were divided into four subgroups based on their levels of expression in fusion cells compared to neighboring non-fusion cells revealed by in situ hybridization: (1) genes that have relative high abundance in fusion cells, (2) genes that are dynamically expressed in fusion cells, (3) genes that have relative low abundance in fusion cells, and (4) genes that are expressed at similar levels in fusion cells and non-fusion tracheal cells. This study identifies the expression profile of fusion cells and hypothetically suggests genes which are necessary for the fusion process and which play roles in distinct stages of fusion, as indicated by the location and timing of expression. These data will provide the basis for a comprehensive understanding of the molecular and cellular mechanisms of branch fusion. (C) 2014 Elsevier B.V. All rights reserved.