ANALYSIS OF ULTRASOUND FIELDS IN CELL CULTURE WELLS FOR IN VITRO ULTRASOUND THERAPY EXPERIMENTS
ANALYSIS OF ULTRASOUND FIELDS IN CELL CULTURE WELLS FOR IN VITRO ULTRASOUND THERAPY EXPERIMENTS
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DOI：
10.1016/j.ultrasmedbio.2011.09.007
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发表时间：
2011-12-01
影响因子：
2.9
通讯作者：
Schmitz, Georg
中科院分区：
文献类型：
--
作者：
Hensel, Karin;Mienkina, Martin P.;Schmitz, Georg
超声是骨折愈合、热疗和实体瘤消融的既定治疗方法。在这一新兴领域，超声进一步用于微泡增强药物递送、基因治疗、声致穿孔和血栓溶解。为了在限定的实验条件下研究选定的治疗效果，设计了用于细胞和组织治疗的体外装置。然而，体外研究往往缺乏重现性和成功转移到其他实验条件。这部分是由于体外实验条件的不确定性。在本文中，超声波在最常见的体外超声波治疗设置细胞培养威尔斯井的传播进行了分析，在模拟和验证水听器测量。用于培养板和生长介质的材料的声学参数被确定。在模拟和测量中揭示了由界面处的反射引起的驻波和环形干涉图案的外观和起源。这导致局部最大压力幅度增加高达5倍。数量的微小变化（e。例如，在一个实施例中，生长培养基体积变化2.56%）以2倍增加或减少细胞层处的峰值稀疏压力。这些压力变化可以在很大程度上影响细胞治疗结果。完全浸没在水浴中的密封细胞培养物提供了最佳的再现性，因此有望获得可转移的治疗结果。（电子邮件：georg. ruhr-uni-bochum.de）（C）2011年世界医学和生物学超声联合会。
Ultrasound is an established therapy method for bone fracture healing, hyperthermia and the ablation of solid tumors. In this new emerging field, ultrasound is further used for microbubble-enhanced drug delivery, gene therapy, sonoporation and thrombolysis. To study selected therapeutic effects in defined experimental conditions, in vitro setups are designed for cell and tissue therapy. However, in vitro studies often lack reproducibility and the successful transfer to other experimental conditions. This is partly because of the uncertainty of the experimental conditions in vitro. In this paper, the ultrasound wave propagation in the most common in vitro ultrasound therapy setups for cell culture wells is analyzed in simulations and verified by hydrophone measurements. The acoustic parameters of the materials used for culture plates and growth media are determined. The appearance and origin of standing waves and ring interference patterns caused by reflections at interfaces is revealed in simulations and measurements. This causes a local maximal pressure amplitude increase by up to the factor of 5. Minor variations of quantities (e. g., growth medium volume variation of 2.56%) increase or decrease the peak rarefaction pressure at a cell layer by the factor of 2. These pressure variations can affect cell therapy results to a large extent. A sealed cell culture well submersed in a water bath provides the best reproducibility and therefore promises transferable therapy results. (E-mail: georg.schmitz@ruhr-uni-bochum.de) (C) 2011 World Federation for Ultrasound in Medicine & Biology.