The development of technetium-99m-labelled interleukin-2: A new radiopharmaceutical for the in vivo detection of mononuclear cell infiltrates in immune-mediated diseases
The development of technetium-99m-labelled interleukin-2: A new radiopharmaceutical for the in vivo detection of mononuclear cell infiltrates in immune-mediated diseases
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DOI：
10.1016/s0969-8051(97)00021-8
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发表时间：
1997-08-01
影响因子：
3.1
通讯作者：
Mather, SJ
中科院分区：
文献类型：
--
作者：
Chianelli, M;Signore, A;Mather, SJ
本文介绍了一种用Tc-99 m标记高比活度白细胞介素-2（IL-2）的新方法，该方法采用N_3S双功能螯合剂通过两步反应进行标记。为了优化反应，研究了影响Tc-99 m掺入N3 S配体的因素。然后类似地优化预先形成的N3 S螯合配体与IL-2的缀合。探讨了用于纯化Tc-99 m-IL-2的各种策略，包括尺寸排阻、离子交换和几种反相色谱模式。用HPLC、ITLC、TCA沉淀和SDS-PAGE测定纯化蛋白的放射化学纯度。研究了Tc-99 m-IL-2的受体结合能力。用不同技术纯化或长期储存后标记的Tc-99 m-IL-2在正常小鼠中进行生物分布研究，并与I-125-IL-2进行比较。(C)1997年爱思唯尔科学公司
We describe here a new method for labelling interleukin-2 (IL-2) in high specific activity with Tc-99m for in vivo studies in man. Labelling was performed via a two-step reaction using an N3S bifunctional chelating agent. To optimise the reaction, factors affecting the incorporation of Tc-99m into the N3S ligand were studied. The conjugation of the preformed N3S chelate ligand to IL-2 was then similarly optimised. Various strategies for purifying the Tc-99m-IL-2 were explored including size exclusion, ion exchange, and several modes of reversed-phase chromatography. The radiochemical purity of the purified protein was determined by HPLC, ITLC, TCA precipitation, and SDS-PAGE. The receptor binding capacity of Tc-99m-IL-2 was studied. Biodistribution studies in normal mice were performed with Tc-99m-IL-2 purified using different techniques or labelled after prolonged storage and compared to I-125-IL-2. (C) 1997 Elsevier Science Inc.