Circadian variations in the liver metabolites of medaka (Oryzias latipes).
Circadian variations in the liver metabolites of medaka (Oryzias latipes).
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Medaka（Oryzias latipes）的肝脏代谢产物中的昼夜节律变化。
DOI：
10.1038/srep20916
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发表时间：
2016-02-10
影响因子：
4.6
通讯作者：
Sakaida I
中科院分区：
文献类型：
--
作者：
Fujisawa K;Takami T;Kimoto Y;Matsumoto T;Yamamoto N;Terai S;Sakaida I
昼夜节律是以24小时为周期的生物节律。在这项研究中，我们比较了青鳉肝脏的代谢组在白天和夜间。为了全面分析肝脏中代谢物水平的昼夜变化，从Zeitgeber时间（ZT）4和ZT 16分离肝脏，并评价代谢物水平的变化。肌苷单磷酸（IMP）和尿苷单磷酸（UMP）在夜间增加，表明核苷酸合成在夜间最活跃。此外，三羧酸循环的代谢物水平也在夜间降低。此外，许多氨基酸的水平在夜间降低，表明氨基酸已被降解。此外，瓜氨酸/鸟氨酸比率与精氨酸消耗量有关，白天比晚上低。这种模式表明，尿素循环在白天被激活，而大量的一氧化氮和瓜氨酸可能在夜间通过一氧化氮合酶由精氨酸产生。这种代谢组学分析的结果可能是有用的，在未来的基础研究，以提供深入了解时间生物学以及应用研究的药物评价青鳉作为模式物种。
Circadian rhythms are biological rhythms with a period of around 24 hours. In this study, we compared the metabolome of the liver of medaka during the day and night. To comprehensively analyze the circadian variations in the levels of metabolites in the liver, livers were isolated from Zeitgeber time (ZT)4 and ZT16, and the variations in metabolite levels were evaluated. Inosinemonophosphate (IMP) and uridinemonophosphate (UMP) were found to be increased at night, indicating that nucleotide synthesis is most active during the night. Furthermore, the levels of metabolites of the tricarboxylic acid cycle were also reduced at night. In addition, the levels of many amino acids were reduced during the night, suggesting that the amino acids had been degraded. Moreover, the citrulline/ornithine ratio, which is related to arginine consumption, was lower during the day than at night. This pattern suggests that the urea cycle is activated during the day, whereas large amounts of nitric oxide and citrulline may be produced from arginine via nitric oxide synthase during the night. The results of this metabolomic analysis may be useful in future fundamental research to provide insight into chronobiology as well as applied research on drug evaluations using medaka as a model species.