Temporal decoding of MAP kinase and CREB phosphorylation by selective immediate early gene expression.
Temporal decoding of MAP kinase and CREB phosphorylation by selective immediate early gene expression.
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DOI：
10.1371/journal.pone.0057037
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Kuroda S
中科院分区：
文献类型：
--
作者：
Saito TH;Uda S;Tsuchiya T;Ozaki Y;Kuroda S
多种生长因子将信息编码为MAP激酶（MAPK）和CREB磷酸化的特定时间模式，这些信息通过即时早期基因产物（eggs）的表达进一步解码，以发挥生物学功能。然而，IEG解码系统仍然是未知的。我们建立了一个基于MAPK和CREB磷酸化和IEG表达响应各种生长因子的时间过程的数据驱动模型，以确定信号是如何被处理的。我们发现IEG表达使用共同的解码系统，而不考虑生长因子和每个IEG的表达在上游依赖性、开关响应和线性时间滤波器方面的差异。脉冲性ERK磷酸化通过表达EGR1而不是c-FOS选择性解码。通过对c-FOS的开关样反应，协同JUNB表达选择性地解码了NGF和PACAP的联合刺激。因此，特定的时间模式和mapk和CREB磷酸化的组合可以通过选择性的IEG表达，通过不同的时间过滤器和开关样反应来解码。数据驱动的建模是通用的信号处理分析，不需要详细的先验知识的途径。
A wide range of growth factors encode information into specific temporal patterns of MAP kinase (MAPK) and CREB phosphorylation, which are further decoded by expression of immediate early gene products (IEGs) to exert biological functions. However, the IEG decoding system remain unknown. We built a data-driven based on time courses of MAPK and CREB phosphorylation and IEG expression in response to various growth factors to identify how signal is processed. We found that IEG expression uses common decoding systems regardless of growth factors and expression of each IEG differs in upstream dependency, switch-like response, and linear temporal filters. Pulsatile ERK phosphorylation was selectively decoded by expression of EGR1 rather than c-FOS. Conjunctive NGF and PACAP stimulation was selectively decoded by synergistic JUNB expression through switch-like response to c-FOS. Thus, specific temporal patterns and combinations of MAPKs and CREB phosphorylation can be decoded by selective IEG expression via distinct temporal filters and switch-like responses. The data-driven modeling is versatile for analysis of signal processing and does not require detailed prior knowledge of pathways.
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