Cell docking inside microwells within reversibly sealed microfluidic channels for fabricating multiphenotype cell arrays
Cell docking inside microwells within reversibly sealed microfluidic channels for fabricating multiphenotype cell arrays
复制标题
DOI：
10.1039/b508096g
复制
发表时间：
2005-01-01
期刊：
影响因子：
6.1
通讯作者：
Langer, R
中科院分区：
文献类型：
--
作者：
Khademhosseini, A;Yeh, J;Langer, R
我们提出了一种软光刻方法，通过捕获可逆密封的微流控通道阵列中的细胞来制造多表型细胞阵列。该技术使用弹性聚二甲基硅氧烷(PDMS)模具在表面上的可逆密封，以顺序地将各种流体或细胞输送到衬底上的特定位置。利用衬底上的微孔捕获和固定通道内低剪应力区的细胞。通过使用一组通道，可以在基质上沉积多种类型的细胞，如肝细胞、成纤维细胞和胚胎干细胞。当在衬底上形成细胞阵列时，PDMS模具可以被移除，从而产生多表型细胞阵列。此外，可以使用在图案化衬底上的二次通道阵列的正交对准和随后的附着来将流体输送到图案化的细胞。在二维底物内的特定区域定位多种细胞类型的能力可能会改进适用于药物筛选和组织工程的高通量方法。
We present a soft lithographic method to fabricate multiphenotype cell arrays by capturing cells within an array of reversibly sealed microfluidic channels. The technique uses reversible sealing of elastomeric polydimethylsiloxane (PDMS) molds on surfaces to sequentially deliver various fluids or cells onto specific locations on a substrate. Microwells on the substrate were used to capture and immobilize cells within low shear stress regions inside channels. By using an array of channels it was possible to deposit multiple cell types, such as hepatocytes, fibroblasts, and embryonic stem cells, on the substrates. Upon formation of the cell arrays on the substrate, the PDMS mold could be removed, generating a multiphenotype array of cells. In addition, the orthogonal alignment and subsequent attachment of a secondary array of channels on the patterned substrates could be used to deliver fluids to the patterned cells. The ability to position many cell types on particular regions within a two dimensional substrate could potentially lead to improved high-throughput methods applicable to drug screening and tissue engineering.