Diffractive oblique plane microscopy
Diffractive oblique plane microscopy
复制标题
DOI：
10.1364/optica.6.001166
复制
发表时间：
2019-09-20
期刊：
影响因子：
10.4
通讯作者：
Judkewitz, Benjamin
中科院分区：
文献类型：
--
作者：
Hoffmann, Maximilian;Judkewitz, Benjamin
用荧光指示剂对神经元活动进行成像是神经科学中的一项重要技术。然而，以快速帧速率和良好分辨率记录体积图像数据仍然具有挑战性。实现这一目标的一项有前景的技术是光片显微镜（LSM），但激发和成像系统的直角配置限制了其应用。斜平面显微镜 (OPM) 是 LSM 的一种变体，它通过激发斜平面并通过相同的显微镜物镜检测图像来规避这一限制。到目前为止，这些技术依赖于使用高数值孔径 (NA) 检测物镜，这限制了它们的视野。在这里，我们提出了一种 OPM 技术，该技术允许通过借助衍射光栅重定向光线来使用低 NA 物镜。该显微镜在 3.3 x 3.0 x 1.0 mm(3) 的大型可寻址成像体积上保持微米级横向分辨率。我们通过对幼虫和幼年斑马鱼的整个大脑进行成像来证明其实用性。 (C) 2019 年美国光学学会根据 OSA 开放获取出版协议条款
Imaging of neuronal activity with fluorescent indicators is an important technique in neuroscience. However, it remains challenging to record volumetric image data at fast frame rates and good resolution. One promising technique to achieve this goal is light sheet microscopy (LSM), but the right angle configuration of the excitation and imaging system limits its application. Oblique plane microscopy (OPM), a variant of LSM, circumvents this limitation by exciting oblique planes and detecting the image through the same microscope objective lens. So far, these techniques have relied on the use of high numerical aperture (NA) detection objective lenses, which limits their field of view. Here we present an OPM technique that allows for the use of low NA objective lenses by redirecting the light with the help of a diffraction grating. The microscope maintains a micrometer-scale lateral resolution over a large addressable imaging volume of 3.3 x 3.0 x 1.0 mm(3). We demonstrate its practicality by imaging the whole brain of larval and juvenile zebrafish. (C) 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement