Transcript analysis of 1003 novel yeast genes using high-throughput northern hybridizations
Transcript analysis of 1003 novel yeast genes using high-throughput northern hybridizations
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DOI：
10.1093/emboj/20.12.3177
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发表时间：
2001-06-15
期刊：
影响因子：
11.4
通讯作者：
Oliver, SG
中科院分区：
文献类型：
--
作者：
Brown, AJP;Planta, RJ;Oliver, SG
利用经典的北方分析方法，在8种不同的生理条件下，检测了1008个来自酿酒酵母的开放阅读框架（ORF）的表达。这些北方的数据进行了比较，与公开可用的数据从微阵列分析的二倍型转换的酿酒酵母。结果表明，比较生物等效的情况和数据标准化程序的标准化的重要性。我们还使用我们的北方数据，以确定共调节基因簇，并确定负责其控制的转录激活因子的推定的靶位点。含有已知功能的基因的簇鉴定已知激活剂的靶位点。相反，仅由未知功能的基因组成的簇通常定义新的推定靶位点。最后，我们研究了基因表达的可能的全局控制。发现在营养上调后高度表达的ORF倾向于采用有利的密码子，而在饥饿条件下过表达的ORF则不采用有利的密码子。这些结果被解释为mRNA分子之间的翻译能力竞争选择由丰富的tRNA翻译的密码子的模型。
The expression of 1008 open reading frames (ORFs) from the yeast Saccharomyces cerevisiae has been examined under eight different physiological conditions, using classical northern analysis. These northern data have been compared with publicly available data from a microarray analysis of the diauxic transition in S, cerevisiae. The results demonstrate the importance of comparing biologically equivalent situations and of the standardization of data normalization procedures. We have also used our northern data to identify co-regulated gene clusters and define the putative target sites of transcriptional activators responsible for their control. Clusters containing genes of known function identify target sites of known activators. In contrast, clusters comprised solely of genes of unknown function usually define novel putative target sites. Finally, we have examined possible global controls on gene expression. It was discovered that ORFs that are highly expressed following a nutritional upshift tend to employ favoured codons, whereas those overexpressed in starvation conditions do not, These results are interpreted in terms of a model in which competition between mRNA molecules for translational capacity selects for codons translated by abundant tRNAs.