Proteomics analysis after traumatic brain injury in rats: the search for potential biomarkers
Proteomics analysis after traumatic brain injury in rats: the search for potential biomarkers
复制标题
大鼠脑外伤后的蛋白质组学分析：寻找潜在的生物标志物
DOI：
10.1590/0004-282x20150006
复制
发表时间：
2015-04-01
影响因子：
1.4
通讯作者：
Tian, Hengli
中科院分区：
文献类型：
--
作者：
Ding, Jun;Ding, Zhengang;Tian, Hengli
Many studies of protein expression after traumatic brain injury (TBI) have identified biomarkers for diagnosing or determining the prognosis of TBI. In this study, we searched for additional protein markers of TBI using a fluid perfusion impact device to model TBI in S-D rats. Two-dimensional gel electrophoresis and mass spectrometry were used to identify differentially expressed proteins. After proteomic analysis, we detected 405 and 371 protein spots within a pH range of 3-10 from sham-treated and contused brain cortex, respectively. Eighty protein spots were differentially expressed in the two groups and 20 of these proteins were identified. This study validated the established biomarkers of TBI and identified potential biomarkers that could be examined in future work.
