Clusterization of nanoparticles during their interaction with living cells
Clusterization of nanoparticles during their interaction with living cells
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DOI：
10.2217/17435889.2.2.241
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发表时间：
2007-04-01
期刊：
影响因子：
5.5
通讯作者：
Oraevsky, Alexander A.
中科院分区：
文献类型：
--
作者：
Lapotko, Dmitri O.;Lukianova-Hleb, Ekaterina Y.;Oraevsky, Alexander A.
目的：纳米颗粒簇可以显着提高诊断的灵敏度以及医疗纳米技术的安全性和有效性。我们报告了形成纳米颗粒簇和监测其在癌细胞中积累的方法。研究方法：采用流式细胞术、光散射和荧光、原子力、光热和扫描电子显微镜研究了金纳米颗粒在肿瘤细胞中的积聚。结果如下：将细胞在37 ℃下与10-30-nm纳米颗粒孵育30分钟或更长时间，导致形成大至20个纳米颗粒或更多的纳米颗粒簇。结论：与非特异性纳米颗粒积聚相比，使用单克隆抗体作为载体的特异性靶向增加了靶细胞表面上纳米颗粒的浓度。反过来，由于内吞作用，靶表面上增加的纳米颗粒浓度在细胞内产生更大的纳米颗粒簇。光热和散射显微镜被认为是最敏感的方法成像纳米粒子簇在活细胞中。
Aims: Clusters of nanoparticles may significantly improve the sensitivity of diagnostics and the safety and efficacy of therapeutic nanotechnologies in medicine. We report methods for the formation of nanoparticle clusters and for monitoring their accumulation in cancer cells. Methods: The accumulation of gold nanoparticles in tumor cells was studied using flow cytometry, optical scattering and fluorescent, atomic force, photothermal and scanning electron microscopy. Results: Incubation of cells at 37 degrees C for 30 min or more with 10-30-nm nanoparticles resulted in the formation of clusters of nanoparticles as large as 20 nanoparticles or more. Conclusions: Specific targeting using a monoclonal antibody as a vector increases the concentration of nanoparticles on the surface of target cells compared with nonspecific nanoparticle accumulation. In turn, an increased concentration of nanoparticles on the target surface yields larger nanoparticle clusters inside the cells due to endocytosis. Photothermal and scattering microscopy were found to be the most sensitive methods for imaging nanoparticle clusters in living cells.