Inhibition of human mononuclear cell proliferation, interleukin synthesis, mRNA for IL-2, IL-6, and leukotriene B4 synthesis by a lipoxygenase inhibitor.
Inhibition of human mononuclear cell proliferation, interleukin synthesis, mRNA for IL-2, IL-6, and leukotriene B4 synthesis by a lipoxygenase inhibitor.
复制标题
脂氧合酶抑制剂抑制人单核细胞增殖、白细胞介素合成、IL-2、IL-6 mRNA 和白三烯 B4 合成。
DOI：
10.1002/jlb.54.4.269
复制
发表时间：
1993
影响因子：
5.5
通讯作者：
Woloschak,GE
中科院分区：
文献类型：
--
作者：
Atluru,D;Gudapaty,S;O'Donnell,MP;Woloschak,GE
Human peripheral blood mononuclear cells (PBMCs) were cultured in vitro with various stimuli and in the presence or absence of a 5‐lipoxygenase (5‐LO) inhibitor, A‐63162, to measure its effects on PBMC proliferation, interleukin‐2 receptor (IL‐2R) expression, interleukin‐2 (IL‐2) synthesis, interleukin‐6 (IL‐6) synthesis, and accumulation of messenger RNA for IL‐2 or IL‐6. A‐63162 inhibited PBMC proliferation stimulated by phytohemagglutinin (PHA) and phorbol myristate acetate (PMA) plus A23187, IL‐2 receptor expression stimulated by PHA, and IL‐2 or IL‐6 synthesis induced by PHA plus PMA or PMA plus A23187. At the same concentration, A‐63162 inhibited accumulation of mRNA for IL‐2 or IL‐6 and also inhibited leukotriene B4(LTB.,) synthesis. Our data indicate that the 5‐LO inhibitor A‐63162 has immunosuppressive activity that may be due to inhibition of LTB4production or to direct inhibitory actions of A‐63162 on IL‐2 and IL‐6 synthesis.
