Isolation of a coaggregation-inhibiting cell wall polysaccharide from Streptococcus sanguis H1
Isolation of a coaggregation-inhibiting cell wall polysaccharide from Streptococcus sanguis H1
复制标题
从血链球菌 H1 中分离抑制共聚集的细胞壁多糖
作者：
Frederick J. CASSELSt;J. London
Coaggregation between Streptococcus sanguis H1 and Capnocytophaga ochracea ATCC 33596 cells is mediated by a carbohydrate receptor on the former and an adhesin on the latter. Two methods were used to release the carbohydrate receptor from the gram-positive streptococcus, autoclaving and mutanolysin treatment. The polysaccharide released from the streptococcal cell wall by either treatment was purified by ion-exchange chromatography; this polysaccharide inhibited coaggregation when preincubated with the gram-negative capnocytophaga partner. After hydrolysis of the polysaccharide by hydrofluoric acid (HF), the major oligosaccharide of the polysaccharide was purified by high-performance liquid chromatography. By analysis of the HF hydrolysis of the polysaccharide and the purified oligosaccharide, this major oligosaccharide appeared to be the repeating unit of the polysaccharide, with minor components resulting from internal hydrolysis of the major oligosaccharide. Gas chromatography results showed that the oligomer was a hexasaccharide, consisting of rhamnose, galactose, and glucose, in the ratio of 2:3:1, respectively. By weight, the purified hexasaccharide was a fourfold-more-potent inhibitor of coaggregation than the native polysaccharide. Resistance to hydrolysis by sulfuric acid alone and susceptibility to hydrolysis by HF suggested that oligosaccharide chains of the polysaccharide are linked by phosphodiester bonds. Studies with a coaggregation-defective mutant of S. sanguis H1 revealed that the cell walls of the mutant contained neither the polysaccharide nor the hexasaccharide repeating unit. The purification of both a polysaccharide and its constituent hexasaccharide repeating unit, which both inhibited coaggregation, and the absence of this polysaccharide or hexasaccharide on a coaggregation-defective mutant strongly suggest that the hexasaccharide derived from the polysaccharide functions as the receptor for the adhesin from C. ochracea ATCC 33596.
影响因子：
3.1
作者：
Warren,CD;Schmit,AS;Jeanloz,RW
通讯作者：
Jeanloz,RW
细菌细胞壁中中性糖和氨基糖的糖醇乙酸酯的毛细管气相色谱分析。
DOI：
10.1016/s0021-9673(01)88260-1
发表时间：
1983
期刊：
Journal of chromatography
影响因子：
--
作者：
Fox,A;Morgan,SL;Hudson,JR;Zhu,ZT;Lau,PY
通讯作者：
Lau,PY
来自血链球菌 34 的共聚多糖的新六糖的结构。
影响因子：
3.1
作者：
McIntire,FC;Bush,CA;Wu,SS;Li,SC;Li,YT;McNeil,M;Tjoa,SS;Fennessey,PV
通讯作者：
Fennessey,PV