PTH-172 Expression patterns of human epidermal growth factor receptor proteins in early gastric cancer
PTH-172 Expression patterns of human epidermal growth factor receptor proteins in early gastric cancer
复制标题
PTH-172人表皮生长因子受体蛋白在早期胃癌中的表达模式
DOI：
10.1136/gutjnl-2015-309861.1060
复制
发表时间：
2015
期刊：
影响因子：
24.5
通讯作者：
Arez M
中科院分区：
文献类型：
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作者：
Arez M
方法对22例早期胃ESD切除术标本进行福尔马林固定石蜡包埋组织切片。正常切除的胃作为对照。优化HER家族受体的免疫组化染色，并对EGFR外部（ED）和内部结构域（ID）、HER2 （ED和ID）、HER3 （ID）和HER4 （ID）进行染色。评分由两名独立观察员进行，并由诊断软件确认。结果2/22（9%）、2/22（9%）和3/22（13%）的患者EGFR ID、HER2 ID和HER2 ED阳性。1/22（4.5%）的病例显示EGFR ID、HER2 ID和HER2 ED共表达，1例显示HER2 ED和ID共表达。EGFR、ED、HER3、HER4均为过表达阴性。HER2 ID （p= 0.047）和HER2 ED （p= 0.0045）的过表达与肿瘤侵袭性呈正相关。与过表达组3/3（100%）相比，无过表达的ESD治疗组10/20（50%）的患者复发异常增生（p= 0.21）。结论：虽然我们的研究规模很小，但我们的研究显示EGFR和HER2表达呈正相关，HER2是治疗的潜在靶点。与报道的相关关系相反，HER3和HER4的表达与任何其他抗体无关。不同抗体克隆的重复将证实这些结果的可靠性。EGFR和HER2共表达预测侵袭性早期胃癌表型的预后效用值得进一步研究。虽然不显著，但HER过表达与ESD治愈率之间的负相关应该在更大的队列中进行随访。
MethodFormalin fixed paraffin embedded tissue from 22 early gastric ESD resection specimens were sectioned. Normal gastric resections were used as a control. Immunohistochemical staining for the HER family receptors were optimised and staining performed against EGFR external (ED) and internal domain (ID), HER2 (ED and ID), HER3 (ID) and HER4 (ID). Scoring was performed by two independent observers and confirmed by diagnostic software.Results2/22 (9%), 2/22 (9%) and 3/22 (13%) of cases were positive for EGFR ID, HER2 ID and HER2 ED respectively. 1/22 (4.5%) of cases showed co-expression of EGFR ID, HER2 ID and HER2 ED. 1 showed HER2 ED and ID co-expression. EGFR ED, HER3 and HER4 were all negative for overexpression. Over-expression of HER2 ID (p= 0.047) and HER2 ED (p= 0.0045) correlated positively with cancer aggressiveness. 10/20 (50%) cases showing no over-expression treated with ESD had reoccurrence of dysplasia, compared to 3/3 (100%) in the over-expressed group (p= 0.21).ConclusionThough small, our study showed a positive relationship between EGFR and HER2 expression, a potential target for therapeutic treatment. HER3 and HER4 expression did not correlate with any other antibody, in contrast to reported relationships. Repeats with different antibody clones would confirm the reliability of these results. The prognostic utility EGFR and HER2 co-expression to predict an aggressive early gastric cancer phenotype warrants further investigation. Although insignificant, the negative correlation between HER over-expression and ESD curative rates should be followed up with a larger cohort.
PTH-175 用于病理分类的拉曼图谱：对速度的需求
DOI：
--
发表时间：
2015
期刊：
影响因子：
--
作者：
O. Old;M. Isabelle;G. Lloyd;C. Kendall;H. Barr;N. Stone
通讯作者：
N. Stone