Complete genome sequences of Clostridium perfringens Del1 strain isolated from chickens affected by necrotic enteritis.
Complete genome sequences of Clostridium perfringens Del1 strain isolated from chickens affected by necrotic enteritis.
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DOI：
10.1186/s13099-017-0217-6
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发表时间：
2017
期刊：
影响因子：
4.2
通讯作者：
Lillehoj HS
中科院分区：
文献类型：
--
作者：
Li C;Yan X;Lillehoj HS
产气荚膜梭菌在自然界中普遍存在。它是动物和人类肠道中的正常居民。作为气性坏疽、坏死和菌血症的主要病原体，C.产气荚膜杆菌引起食物中毒、坏死性肠炎（NE），甚至死亡。流行病学研究表明，家禽中NE发病率的增加与政府法规要求家禽生产中停用饲料中抗生素生长促进剂有关。最近的组学研究表明，细菌的毒力通常与毒素的高效接合转移或携带抗药性性状的质粒有关。目前，关于C.产气荚膜杆菌阐明这种发病机制对于通过采取预防或治疗干预策略来防治传染病具有实际影响。本研究对中国对虾的基因组进行了测序分析。使用PacBio和Illumina测序技术的混合物对产气荚膜杆菌Del 1菌株进行测序。序列分析表明，Del 1株含有一条环状染色体，全长3，559，163 bp，含有4个质粒：pDel1_1（82，596 bp）、pDel1_2（69，827 bp）、pDel1_3（49，582 bp）和pDel1_4（49，728 bp）。该基因组有3361个预测编码DNA序列，含有许多致病和毒力因子基因，包括6个抗生素和抗菌素耐药性基因和3个噬菌体编码基因。系统进化分析表明，Del 1株与CP 4株基因组和质粒序列相似。本文报道了Del 1菌株的染色体和质粒全序列。由于Del 1是从田间疾病爆发中分离的，因此该菌株是鉴定引起鸡肠道梭菌感染的许多破坏性影响的毒力基因的良好来源。从商业化养殖场分离的鸡致病性分离株的基因组测序为研究C.产气荚膜杆菌是食用动物的胃肠道病原体。该重要野毒株基因序列的详细信息将有助于开发新的C.在鸡中产气荚膜杆菌诱导的NE。本文的在线版本（10.1186/s13099-017-0217-6）包含补充材料，可供授权用户使用。
Clostridium perfringens is ubiquitous in nature. It is a normal inhabitant in the intestinal tract of animals and humans. As the primary etiological agent of gas gangrene, necrosis and bacteremia, C. perfringens causes food poisoning, necrotic enteritis (NE), and even death. Epidemiology research has indicated that the increasing incidence of NE in poultry is associated with the withdrawal of in-feed antibiotic growth promoters in poultry production in response to government regulations. The recent omics studies have indicated that bacterial virulence is typically linked to highly efficient conjugative transfer of toxins, or plasmids carrying antibiotic-resistance traits. Currently, there is limited information on understanding of host–pathogen interaction in NE caused by virulent strains of C. perfringens. Elucidating such pathogenesis has practical impacts on fighting infectious diseases through adopting strategies of prophylactic or therapeutic interventions. In this report, we sequenced and analyzed the genome of C. perfringens Del1 strain using the hybrid of PacBio and Illumina sequencing technologies. Sequence analysis indicated that Del1 strain comprised a single circular chromosome with a complete 3,559,163 bp and 4 plasmids: pDel1_1 (82,596 bp), pDel1_2 (69,827 bp), pDel1_3 (49,582 bp), and pDel1_4 (49,728 bp). The genome had 3361 predicted coding DNA sequences, harbored numerous genes for pathogenesis and virulence factors, including 6 for antibiotic and antimicrobial resistance, and 3 phage-encoded genes. Phylogenetic analysis revealed that Del1 strain had similar genome and plasmid sequences to the CP4 strain. Complete chromosomal and plasmid sequences of Del1 strain are presented in this report. Since Del1 was isolated from a field disease outbreak, this strain is a good source to identify virulent genes that cause many damaging effects of Clostridial infections in chicken gut. Genome sequencing of the chicken pathogenic isolates from commercial farms provides valuable insights into the molecular pathogenesis of C. perfringens as a gastrointestinal pathogen in food animals. The detailed information on gene sequencing of this important field strain will benefit the development of novel vaccines specific for C. perfringens-induced NE in chickens. The online version of this article (10.1186/s13099-017-0217-6) contains supplementary material, which is available to authorized users.