Post-capture immune gene expression studies in the deep-sea hydrothermal vent mussel Bathymodiolus azoricus acclimatized to atmospheric pressure
Post-capture immune gene expression studies in the deep-sea hydrothermal vent mussel Bathymodiolus azoricus acclimatized to atmospheric pressure
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DOI：
10.1016/j.fsi.2014.10.018
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发表时间：
2015-01-01
影响因子：
4.7
通讯作者：
Bettencourt, Raul
中科院分区：
文献类型：
--
作者：
Barros, Ines;Divya, Baby;Bettencourt, Raul
深海热液喷口是分布在世界各地的极端生境，与火山和构造事件有关，因此形成了特殊的环境条件，在这种环境条件下，高压、陡峭的温度梯度以及硫磺、甲烷和重金属的潜在有毒浓度构成了以化学合成为基础的生态系统的驱动因素。在深海热液喷口发现的所有不同的大型生物中，蓝贻贝是生活在大西洋中脊(MAR)喷口生态系统中最丰富的物种。本研究从生理适应的角度研究了大气压长期驯化对寄主共生关系的影响，并利用实时荧光定量聚合酶链式反应对寄主共生体和免疫共生体的基因表达进行了定量研究。与时间表达研究中测试的其他时间点相比，宿主免疫和细菌基因的基因信号分布在驯化24小时、1周和3周时遵循基因表达的阶段性变化。对细菌基因表达的分析也表明，细菌密度和活性都有助于形成内共生菌和宿主免疫基因之间的复杂关联，它们的表达模式在驯化1周后似乎是伴随的。利用荧光原位杂交法研究了内共生菌在鳃组织中的分布和分布，证实了硫氧化(SOX)和甲烷氧化(MOX)细菌在驯化过程中逐渐丧失。本研究以深海喷口贻贝B.azoricus为模式生物，研究水族箱驯化和共生菌的流行是如何驱动宿主免疫基因表达的。到目前为止还没有发现的紧密联系表明，宿主免疫和细菌基因表达模式反映了在水族馆条件下驯化过程中不同的生理反应。(C)2014爱思唯尔有限公司。保留所有权利。
Deep-sea hydrothermal vents are extreme habitats that are distributed worldwide in association with volcanic and tectonic events, resulting thus in the establishment of particular environmental conditions, in which high pressure, steep temperature gradients, and potentially toxic concentrations of sulfur, methane and heavy metals constitute driving factors for the foundation of chemosynthetic-based ecosystems. Of all the different macroorganisms found at deep-sea hydrothermal vents, the mussel Bathy-modiolus azoricus is the most abundant species inhabiting the vent ecosystems from the Mid-Atlantic Ridge (MAR). In the present study, the effect of long term acclimatization at atmospheric pressure on host symbiotic associations were studied in light of the ensuing physiological adaptations from which the immune and endosymbiont gene expressions were concomitantly quantified by means of real-time PCR.The expression of immune genes at 0 h, 12 h, 24 h, 36 h, 48 h, 72 h, 1 week and 3 weeks post-capture acclimatization was investigated and their profiles compared across the samples tested. The gene signal distribution for host immune and bacterial genes followed phasic changes in gene expression at 24 h, 1 week and 3 weeks acclimatization when compared to other time points tested during this temporal expression study. Analyses of the bacterial gene expression also suggested that both bacterial density and activity could contribute to shaping the intricate association between endosymbionts and host immune genes whose expression patterns seem to be concomitant at 1 week acclimatization. Fluorescence in situ hybridization was used to assess the distribution and prevalence of endosymbiont bacteria within gill tissues confirming the gradual loss of sulfur-oxidizing (SOX) and methane-oxidizing (MOX) bacteria during acclimatization.The present study addresses the deep-sea vent mussel B. azoricus as a model organism to study how acclimatization in aquaria and the prevalence of symbiotic bacteria are driving the expression of host immune genes. Tight associations, unseen thus far, suggest that host immune and bacterial gene expression patterns reflect distinct physiological responses over the course of acclimatization under aquarium conditions. (C) 2014 Elsevier Ltd. All rights reserved.