Mechanisms mediating the antiproliferative effects of nitric oxide in cultured human airway smooth muscle cells
Mechanisms mediating the antiproliferative effects of nitric oxide in cultured human airway smooth muscle cells
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DOI：
10.1016/s0014-5793(01)02883-6
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发表时间：
2001-10-05
期刊：
影响因子：
3.5
通讯作者：
Knox, AJ
中科院分区：
文献类型：
--
作者：
Hamad, AM;Knox, AJ
我们已经确定了NO在人气道平滑肌细胞（HASMC）中的抗增殖作用的机制。一氧化氮供体S-亚硝基-N-乙酰青霉胺在细胞周期的G（1）和S期均抑制增殖。此外，用8-溴-cGMP、血红蛋白（一种NO清除剂）和扎普司特（一种cGMP特异性磷酸二酯酶抑制剂）进行的实验表明，这两种作用都是NO介导的。G（1）期抑制是cGMP依赖性的，而S期抑制是由于核苷酸还原酶的cGMP非依赖性抑制。这些结果表明，NO抑制HASMC增殖的cGMP依赖性和非依赖性的机制，在细胞周期中的不同点。(C)2001年欧洲生物化学学会联合会。由Elsevier Science B. V.出版，版权所有。
We have characterised the mechanisms involved in the antiproliferative effect of NO in human airway smooth muscle cells (HASMC). S-Nitroso-N-acetyl penicillamine, a nitric oxide donor, inhibited proliferation in both G(1) and S phases of the cell cycle. Additionally, experiments with 8-bromo-cGMP, haemoglobin, a NO scavenger and zaprinast, a cGMP-specific phosphodiesterase inhibitor, showed that both effects were NO-mediated. The G(1) phase inhibition was cGMP-dependent whereas the S phase inhibition was due to a cGMP-independent inhibition of ribonucleotide reductase. These results demonstrate that NO inhibits HASMC proliferation by cGMP-dependent and -independent mechanisms acting at distinct points in the cell cycle. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.