Systematic investigation of porogen size and content on scaffold morphometric parameters and properties
Systematic investigation of porogen size and content on scaffold morphometric parameters and properties
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DOI：
10.1021/bm061139q
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发表时间：
2007-05-01
影响因子：
6.2
通讯作者：
Cicerone, Marcus T.
中科院分区：
文献类型：
--
作者：
Lin-Gibson, Sheng;Cooper, James A.;Cicerone, Marcus T.
对光聚合二甲基丙烯酸酯盐析法制备的组织工程支架进行了系统的研究，以确定支架的结构(孔隙率、孔径等)。可以控制，还可以确定支架结构和机械性能之间的关系。制备了两个系列的支架：(1)相同的聚合物与盐的比例，但不同的盐尺寸(平均尺寸为100~390微米)和(2)相同的盐尺寸，但不同的聚合物与盐的比例(从70%到90%的盐质量)。对这些支架进行了检查，以确定制造参数如何影响支架的形态计量参数和相应的力学性能。采用X射线微电子计算机断层扫描(MU CT)、压汞测孔仪和重量分析相结合的方法，测定了支架的孔隙率、孔径大小、支架厚度及其尺寸分布、孔的连通性等参数。利用目前的技术可以制备具有互联结构的孔隙率在57%到92%(体积比)之间的支架。随后，孔隙率和支柱厚度与支架的机械响应有关，这两个参数都对支架的压缩弹性系数有贡献。目前的研究表明，支架的结构和性能可以通过支架制造中使用的致孔剂的大小和数量来定制。
A systematic investigation of tissue engineering scaffolds prepared by salt leaching of a photopolymerized dimethacrylate was performed to determine how the scaffold structure (porosity, pore size, etc.) can be controlled and also to determine how the scaffold structure and the mechanical properties are related. Two series of scaffolds were prepared with (1) the same polymer-to-salt ratio but different salt sizes (ranging from average size of 100 to 390 mu m) and (2) the same salt size but different polymer-to-salt ratios (ranging from salt mass of 70 to 90%). These scaffolds were examined to determine how the fabrication parameters affected the scaffold morphometric parameters and corresponding mechanical properties. Combined techniques of X-ray microcomputed tomography (mu CT), mercury porosimetry, and gravimetric analysis were used to determine the scaffold parameters, such as porosity, pore size, and strut thickness and their size distributions, and pore interconnectivity. Scaffolds with porosities ranging from 57% to 92% (by volume) with interconnected structures could be fabricated using the current technique. The porosity and strut thickness were subsequently related to the mechanical response of the scaffolds, both of which contribute to the compression modulus of the scaffold. The current study shows that the structure and properties of the scaffold could be tailored by the size and the amount of porogen used in the fabrication of the scaffold.