A Highly Photostable Near-Infrared Labeling Agent Based on a Phospha-rhodamine for Long-Term and Deep Imaging
A Highly Photostable Near-Infrared Labeling Agent Based on a Phospha-rhodamine for Long-Term and Deep Imaging
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DOI：
10.1002/anie.201804731
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发表时间：
2018-08-06
影响因子：
16.6
通讯作者：
Yamaguchi, Shigehiro
中科院分区：
文献类型：
--
作者：
Grzybowski, Marek;Taki, Masayasu;Yamaguchi, Shigehiro
各种荧光显微技术要求发光的近红外荧光团具有高的化学稳定性和光稳定性。现已有报道，含磷取代罗丹明染料(PORS)在9位取代2，6-二甲氧基苯基后，性能有了显著的提高。这样得到的染料Prex 710被用来对活细胞中的线粒体进行染色，从而可以在可见-近红外范围内进行长期的三色成像。此外，Prex 710的高荧光寿命允许在生理条件下通过单分子显微镜跟踪染料标记的生物分子。使用明亮的近红外发射的Prex 710-葡聚糖偶联物，还可以对小鼠大脑中的血管进行深度成像。
Various fluorescence microscopy techniques require bright NIR-emitting fluorophores with high chemical and photostability. Now, the significant performance improvement of phosphorus-substituted rhodamine dyes (PORs) upon substitution at the 9-position with a 2,6-dimethoxyphenyl group is reported. The thus obtained dye PREX 710 was used to stain mitochondria in living cells, which allowed long-term and three-color imaging in the vis-NIR range. Moreover, the high fluorescence longevity of PREX 710 allows tracking a dye-labeled biomolecule by single-molecule microscopy under physiological conditions. Deep imaging of blood vessels in mice brain has also been achieved using the bright NIR-emitting PREX 710-dextran conjugate.