IMMUNOELECTRON MICROSCOPIC DETECTION OF ESTROGEN TARGET-CELLS IN THE BONE-MARROW OF ESTROGEN-TREATED MALE JAPANESE-QUAIL
IMMUNOELECTRON MICROSCOPIC DETECTION OF ESTROGEN TARGET-CELLS IN THE BONE-MARROW OF ESTROGEN-TREATED MALE JAPANESE-QUAIL
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DOI：
10.1016/s0169-6009(08)80035-9
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发表时间：
1993-01-01
期刊：
影响因子：
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通讯作者：
KUSUHARA, S
中科院分区：
文献类型：
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作者：
OHASHI, T;KUSUHARA, S
用免疫电镜技术研究了雌激素处理雄性日本鹌鹑股骨骨髓细胞雌激素受体（ER）的定位。雄鹌鹑骨髓细胞中未检测到ER定位的电子致密反应产物。雌激素处理48 h后，骨髓单个核细胞中可见核反应产物。大多数位于骨髓基质中，分为未分化型和分化型细胞，分别以胞质不发达和发达为特征。部分ER阳性细胞位于造血组织中，胞质中等发达。因此，雌激素靶细胞可能存在于骨髓的基质和造血组织中。
The localization of estrogen receptors (ERs) in bone marrow cells was studied immunoelectron microscopically in the femurs of estrogen-treated male Japanese quail. The electron-dense reaction product showing ER localization was not detected in the bone marrow cells of the male quail. After 48 h of estrogen treatment, nuclear reaction product was observed in many mononuclear cells in the bone marrow. Most were located in the marrow stroma, and they were divided into undifferentiated and differentiated type cells, characterized by poorly and well-developed cytoplasm, respectively. Some ER-positive cells were situated in the hematopoietic tissue and had moderately developed cytoplasm. Thus, estrogen target cells may be present in the stroma and hematopoietic tissue of bone marrow.