Single-particle chemical force microscopy to characterize virus surface chemistry
Single-particle chemical force microscopy to characterize virus surface chemistry
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DOI：
10.2144/btn-2020-0085
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发表时间：
2020-10-01
期刊：
影响因子：
2.7
通讯作者：
Heldt, Caryn L.
中科院分区：
文献类型：
--
作者：
Mi, Xue;Heldt, Caryn L.
病毒的两个重要表面特性是疏水性和表面电荷，它们决定了病毒的胶体行为和流动性。化学力显微镜可以在含有少量病毒样品的液体样品中检测病毒表面化学。这种单粒子方法需要原子力显微镜（AFM）探针的功能化和病毒与表面的共价键。用疏水甲基修饰的AFM探针研究病毒表面的疏水性，用带负电的羧酸或带正电的季胺末端的AFM探针研究病毒表面的电荷。随着对病毒表面特性的了解，病毒与环境相互作用的方式可以更好地预测。方法总结：我们建立了一种化学力显微镜技术，采用简单的探针功能化方法，利用硫醇附着和优化的共价病毒固定方法。这种新方法被用于检测病毒衣壳在单粒子水平上的表面电荷和疏水性。
Two important viral surface characteristics are the hydrophobicity and surface charge, which determine the viral colloidal behavior and mobility. Chemical force microscopy allows the detection of viral surface chemistry in liquid samples with small amounts of virus sample. This single-particle method requires the functionalization of an atomic force microscope (AFM) probe and covalent bonding of viruses to a surface. A hydrophobic methyl-modified AFM probe was used to study the viral surface hydrophobicity, and an AFM probe terminated with either negatively charged carboxyl acid or positively charged quaternary amine was used to study the viral surface charge. With an understanding of viral surface properties, the way in which viruses interact with the environment can be better predicted.METHOD SUMMARY We developed a chemical force microscopy technique with a simple probe functionalization method using thiol attachment and an optimized covalent virus immobilization method. The novel method was used to detect the surface charge and hydrophobicity of viral capsids at a single-particle level.