A molecular phylogeny of Solanum sect. Pteroidea (Solanaceae) and the utility of COSII markers in resolving relationships among closely related species

A molecular phylogeny of Solanum sect. Pteroidea (Solanaceae) and the utility of COSII markers in resolving relationships among closely related species
复制标题

茄科的分子系统发育。

DOI：
--
复制
发表时间：
2010
期刊：
影响因子：
--
通讯作者：
L. Bohs
L. Bohs
中科院分区：
--
文献类型：
--
作者：
E. Tepe;L. Bohs

文献摘要

被引文献

相似文献

茄科。蕨总科是十种新热带草本植物和藤本植物的谱系，分布中心位于安第斯山脉东部斜坡。它是 Solanum 马铃薯分支的成员，该分支包括马铃薯 (S. tuberosum) 和番茄 (S. lycopersicum)。 S.教派成员。大多数种类的蕨类植物的特征是从叶腋长出的花序和有皱纹、尖尖的果实。本研究的目的是推断茄属植物 16 个物种之间的系统发育关系，其中包括茄科所有 10 个物种。 Pteroidea，使用来自叶绿体 trnT-trnF 和五个核区域的 DNA 序列数据：ITS、颗粒结合淀粉合酶基因（GBSSI 或蜡）和三个保守直系同源集 II (COSII) 标记。结果为S. sect的单系性提供了强有力的支持。 Pteroidea 及其与 S. sec 的姐妹群关系。疱疹属。茄螨是该科中分布最广、形态变化最广泛的物种之一，它不是单系的。使用全证据串联方法和 BEST 中实施的合并方法进行贝叶斯分析，产生了基本一致的拓扑。然而，总证据树比最佳树得到的支持要高得多。虽然在 S. 教派中单独使用并不有用。在翼总科中，三个 COSII 标记易于扩增，提供了清晰的序列，并且对于提高翼总科密切相关物种之间的分辨率和支持非常有用。综合分析中的翼总目。
Solanum sect. Pteroidea is a lineage of ten species of neotropical herbs and vines with a center of distribution in the eastern Andean slopes. It is a member of the Potato clade of Solanum, a group that includes the potato (S. tuberosum) and tomato (S. lycopersicum). Members of S. sect. Pteroidea are characterized by inflorescences that emerge from the leaf axils and rugose, sharply pointed fruits in most species. The aim of this study is to infer phylogenetic relationships among sixteen species of Solanum, including all ten species of S. sect. Pteroidea, using DNA sequence data from the chloroplast trnT-trnF and five nuclear regions: ITS, the granule bound starch synthase gene (GBSSI or waxy), and three Conserved Orthologous Set II (COSII) markers. Results provide strong support for the monophyly of S. sect. Pteroidea and for its sister group relationship to S. sect. Herpystichum. Solanum mite, one of the most widespread and morphologically variable species in the section, is not monophyletic. Bayesian analyses using a total evidence concatenated approach and a coalescent approach implemented in BEST produced largely congruent topologies. The total evidence trees, however, were much more highly supported than the BEST trees. Although not useful individually in S. sect. Pteroidea, the three COSII markers were easy to amplify, provided clean sequences, and were tremendously useful in increasing resolution and support among the closely related species of S. sect. Pteroidea in combined analyses.