Fish Protein Decreases Serum Cholesterol in Rats by Inhibition of Cholesterol and Bile Acid Absorption
Fish Protein Decreases Serum Cholesterol in Rats by Inhibition of Cholesterol and Bile Acid Absorption
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DOI：
10.1111/j.1750-3841.2011.02130.x
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发表时间：
2011-05-01
影响因子：
3.9
通讯作者：
Yoshida, Munehiro
中科院分区：
文献类型：
--
作者：
Hosomi, Ryota;Fukunaga, Kenji;Yoshida, Munehiro
鱼蛋白已被证明可以通过抑制实验动物对胆固醇和胆汁酸的吸收来降低血清胆固醇含量，尽管这种作用的机制尚未完全清楚。本研究的目的是阐明摄入鱼蛋白后抑制胆固醇和胆汁酸吸收的机制。将雄性Wistar大鼠分为2个饮食组，每组7只大鼠，一组接受由20%酪蛋白组成的饮食，另一组接受由10%酪蛋白和10%鱼蛋白组成的饮食。两种实验饮食还含有 0.5% 胆固醇和 0.1% 胆酸钠。大鼠分别进食4周后，测定血清和肝脏胆固醇含量以及粪便胆固醇、胆汁酸和氮排泄含量。鱼蛋白的消耗降低了血清和肝脏胆固醇含量，增加了粪便胆固醇和胆汁酸的排泄，同时增加了粪便氮的排泄。此外，与酪蛋白水解物相比，体外消化制备的鱼蛋白水解物具有较低的胆固醇胶束溶解度和较高的胆汁酸结合能力。这些结果表明，鱼蛋白的降胆固醇作用是通过增加粪便胆固醇和胆汁酸排泄来介导的，这是由于鱼蛋白的消化产物降低了胆固醇的胶束溶解度并增加了胆汁酸结合能力。 实际应用鱼蛋白降低了血清和肝脏胆固醇含量，增加了粪便胆固醇和胆汁酸的排泄，同时增加了粪便氮的排泄。此外，与酪蛋白水解物相比，体外消化制备的鱼蛋白水解物具有较低的胆固醇胶束溶解度和较高的胆汁酸结合能力。这些结果表明，鱼蛋白的降胆固醇作用是通过增加粪便胆固醇和胆汁酸排泄来介导的，这是由于鱼蛋白消化产物的胆固醇胶束溶解度降低和胆汁酸结合能力增加所致。摄入鱼蛋白可有效改善血液胆固醇水平，可能对高胆固醇血症患者有益。根据这项研究的结果，我们得出的结论是，可以向此类患者推荐功能强大的替代饮食，作为促进健康的一种手段。
Fish protein has been shown to decrease serum cholesterol content by inhibiting absorption of cholesterol and bile acid in laboratory animals, though the mechanism underlying this effect is not yet fully understood. The purpose of this study was to elucidate the mechanism underlying the inhibition of cholesterol and bile acid absorption following fish protein intake. Male Wistar rats were divided into 2 dietary groups of 7 rats each, 1 group receiving a diet consisting of 20% casein and the other receiving a diet consisting of 10% casein and 10% fish protein. Both experimental diets also contained 0.5% cholesterol and 0.1% sodium cholate. After the rats had been on their respective diets for 4 wk, their serum and liver cholesterol contents and fecal cholesterol, bile acid, and nitrogen excretion contents were measured. Fish protein consumption decreased serum and liver cholesterol content and increased fecal cholesterol and bile acid excretion and simultaneously increased fecal nitrogen excretion. In addition, fish protein hydrolyzate prepared by in vitro digestion had lower micellar solubility of cholesterol and higher binding capacity for bile acids compared with casein hydrolyzate. These results suggest that the hypocholesterolemic effect of fish protein is mediated by increased fecal cholesterol and bile acid excretion, which is due to the digestion products of fish protein having reduced micellar solubility of cholesterol and increased bile acid binding capacity.Practical ApplicationFish protein decreased serum and liver cholesterol content and increased fecal cholesterol and bile acid excretion and simultaneously increased fecal nitrogen excretion. In addition, fish protein hydrolyzate prepared by in vitro digestion had lower micellar solubility of cholesterol and higher binding capacity for bile acids compared with casein hydrolyzate. These results suggest that the hypocholesterolemic effect of fish protein is mediated by increased fecal cholesterol and bile acid excretion, which is due to the reduced micellar solubility of cholesterol and increased bile acid binding capacity of the digestion products of fish protein. Fish protein intake is effective for improving blood cholesterol level and may have a beneficial effect for hypercholesterolemia patients. We conclude that a highly functional alternative diet could be recommended for such patients as a means of promoting health, based on the results of this study.