MOLECULAR ANALYSIS OF THE YELLOW GENE (Y) REGION OF DROSOPHILA-MELANOGASTER
MOLECULAR ANALYSIS OF THE YELLOW GENE (Y) REGION OF DROSOPHILA-MELANOGASTER
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DOI：
10.1073/pnas.82.21.7369
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发表时间：
1985-01-01
影响因子：
11.1
通讯作者：
BIESSMANN, H
中科院分区：
文献类型：
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作者：
BIESSMANN, H
黄色基因（y）与果蝇成虫和果蝇幼虫口器表皮的模式特异性黑色素沉着有关。黑腹菌我已经从y区域分离了大约70千碱基（kb）的连续DNA。染色体畸变的y1型等位基因（无效等位基因）和y2型等位基因引起的特征模式镶嵌的色素沉着进行了定位Southern印迹分析。Y2等位基因与“吉普赛”转座因子在推定的Y编码区远端0.9 kb处的插入相关。一个3.1 kb的区域，其中所有的y1型等位基因的断点可以映射到一个2.0 kb的多聚腺苷酸化的mRNA，其表达是专门调节在发展中同源。这种假定的y基因转录本是目前在高水平的蛹时，黑化的成人角质层发生，但其稳态水平的变化显着的发展过程中，是最高的晚期胚胎孵化前。这表明，除了黑色素合成和/或沉积，y基因产物可能在其他可能的神经功能中发挥作用。
The yellow gene (y) is involved in pattern-specific melanin pigmentation of the cuticle of the adult fly and of larval mouthparts of D. melanogaster. I have isolated some 70 kilobases (kb) of contiguous DNA from the y region. Chromosomal aberratons of y1-type alleles (null alleles) and y2-type alleles that give rise to characteristic pattern mosaicism of pigmentation were mapped by Southern blot analysis. The y2 allele is associated with the insertion of a "gypsy" transposable element 0.9 kb distal to the putative y coding region. A 3.1-kb region to which breakpoints of all y1-type alleles could be mapped is homologous to a 2.0-kb polyadenylylated mRNA, the expression of which is specifically regulated in development. This putative y gene transcript is present at high levels in pupae when melanization of the adult cuticle occurs, but its steady-state levels change dramatically during development, being highest in late embryos prior to hatching. This suggests that, in addition to melanin synthesis and/or deposition, the y gene product may have a role in other possibly neural functions.