Assessment of Plasmodium falciparum Antigen-Specific B Cells.
Assessment of Plasmodium falciparum Antigen-Specific B Cells.
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恶性疟原虫抗原特异性 B 细胞的评估。
DOI：
10.1007/978-1-0716-2189-9_52
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
Crompton,PeterDobbs
中科院分区：
文献类型：
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作者：
Hopp,ChristineSarah;Taylor,JustinJames;Crompton,PeterDobbs
该方案描述了利用B细胞受体和同源抗原之间结合的特异性来检测和表征疟原虫特异性人B细胞的方法。重要的是，这种方法允许分离和研究B细胞，而不需要激活细胞或需要它们分泌抗体。该方案描述了如何生成抗原“探针”并将其用于流式细胞术中以鉴定和分选抗原特异性B细胞，并包括在流式细胞术之前富集抗原特异性B细胞的方法。最后，我们详细介绍了技术，以优化排除B细胞是不特定的抗原的兴趣。
This protocol describes methods that exploit the specificity of binding between the B cell receptor and cognate antigen to detect and characterizePlasmodium-specific human B cells. Importantly, this approach allows for the isolation and study of B cells without activating the cells or requiring them to secrete antibodies. The protocol describes how antigen “probes” are generated and used in flow cytometry to identify and sort antigen-specific B cells, and includes methods for enrichment of antigen-specific B cells prior to flow cytometry. Finally, we detail techniques to optimize the exclusion of B cells that are not specific for the antigen of interest.