Abstract 5176: Cell free malignant ascites fluid facilitates gastric adenocarcinoma peritoneal metastasis
Abstract 5176: Cell free malignant ascites fluid facilitates gastric adenocarcinoma peritoneal metastasis
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摘要 5176：无细胞恶性腹水促进胃腺癌腹膜转移
作者：
Chantelle A. Janeiro;Vivian Stavrakos;Malak Alzahrani;R. Rayes;B. Giannias;N. Bertos;V. Sangwan;J. Cools;J. Spicer;L. Ferri
胃腺癌是北美增长最快的恶性肿瘤。它通常与恶性腹水（MA）有关，恶性腹水是腹膜中含有癌细胞的液体的病理性积聚。腹膜转移是根治性手术后胃癌（GC）进展的最常见部位，也是死亡的主要原因。在腹膜扩散后，恶性过程被认为是不可治愈的，因为它很少适合手术切除，化疗方案只是姑息性的。我们推测无细胞MA增加了GC细胞恶化腹膜转移的可能性。将胃腺癌细胞系（人：MKN-45、SNU-5、KATO III、OKAJIMA;鼠NCC-S1、NCC-S1 M）与无细胞MA一起孵育，并通过静态体外粘附试验以及迁移试验评估其转移能力。一种新的离体腹膜转移模型进一步证实了体外结果，其中通过与非刺激和预刺激的癌细胞共孵育来评估癌细胞对剥离的人腹膜的粘附。在体内腹膜内和脾内注射后数周内监测大体肝转移，由此用对照培养基或刺激的NCC-S1 M细胞接种C57 BL/6小鼠。人GC细胞和/或人腹膜间皮细胞（HPMC）与无细胞MA的孵育导致与未刺激条件相比GC细胞与HPMC的粘附显著增加三至五倍（P引文格式：Chantelle A.放大图片创作者：Janeiro，Vivian Stavrakos. Rayes，France Bourdeau，Betty Giannias，Nicholas Bertos，Veena Sangwan，Jonathan Cools-Lartigue，Jonathan D.放大图片作者：斯派塞.费里无细胞恶性腹水促进胃腺癌腹膜转移[摘要]。在：2018年美国癌症研究协会年会论文集; 2018年4月14日至18日;芝加哥，IL。Philadelphia（PA）：AACR; Cancer Res 2018;78（13 Suppl）：Abstract nr 5176.
Gastric adenocarcinoma is the fastest rising malignancy in North America. It is commonly associated with malignant ascites (MA), the pathological accumulation of fluid containing cancer cells in the peritoneum. Peritoneal metastasis is the most common site of gastric cancer (GC) progression after curative intent surgery and is the leading cause of death. Upon peritoneal dissemination, the malignant process is deemed non-curative as it is rarely amenable to surgical resection and chemotherapeutic regimens are simply palliative. We hypothesize that cell-free MA increases the potential of GC cells to worsen peritoneal metastasis. Gastric adenocarcinoma cell lines (human: MKN-45, SNU-5, KATO III, OKAJIMA; murine NCC-S1, NCC-S1M) were incubated with cell-free MA and their metastatic ability assessed with static in vitro adhesion assays, as well as migration assays. A novel ex vivo peritoneal metastasis model further corroborated the in vitro results, where cancer cell adhesion to stripped human peritoneum was assessed by co-incubation with non- and pre-stimulated cancer cells. Gross liver metastasis was monitored over several weeks after in vivo intra-peritoneal and intra-splenic injections, whereby C57BL/6 mice were inoculated with control media or stimulated NCC-S1M cells. Incubation of human GC cells and/or human peritoneal mesothelial cells (HPMC) with cell-free MA resulted in a significant three to five-fold increase of GC cell adhesion to HPMC compared to non-stimulated condition (P Citation Format: Chantelle A. Janeiro, Vivian Stavrakos, Malak Alzahrani, Roni F. Rayes, France Bourdeau, Betty Giannias, Nicholas Bertos, Veena Sangwan, Jonathan Cools-Lartigue, Jonathan D. Spicer, Lorenzo E. Ferri. Cell free malignant ascites fluid facilitates gastric adenocarcinoma peritoneal metastasis [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2018; 2018 Apr 14-18; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2018;78(13 Suppl):Abstract nr 5176.