Human adipose and skeletal muscle tissue DNA, RNA, and protein content.
Human adipose and skeletal muscle tissue DNA, RNA, and protein content.
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人体脂肪和骨骼肌组织 DNA、RNA 和蛋白质含量。
DOI：
10.1152/japplphysiol.00343.2021
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
Trappe,ScottW
中科院分区：
文献类型：
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作者：
Stroh,AndrewM;Lynch,ColleenE;Lester,BridgetE;Minchev,Kiril;Chambers,TobyL;Montenegro,CristhianF;ChavezMartinez,Clarisa;Fountain,WilliamA;Trappe,ToddA;Trappe,ScottW
该项目的目的是提供脂肪组织中DNA， RNA和蛋白质含量的概况，这在人类中研究相对较少，以更深入地了解各种分析可能需要的组织数量。骨骼肌组织也进行了研究，以提供对这两种高度代谢活跃组织之间潜在差异的直接比较。基础脂肪和骨骼肌组织样本来自10名（7米，3米）娱乐活动参与者[25±1年；84±3kg，最大耗氧量（V * o2max）： 3.5±0.2 L/min，体脂：29±2%。提取DNA、RNA和蛋白质，随后分析其数量和质量。脂肪和骨骼肌组织DNA含量分别为52±14和189±44 ng DNA·mg tissue−1 （P< 0.05）。脂肪和骨骼肌组织RNA含量分别为46±14和537±72 ng RNA·mg tissue−1 （P< 0.05）。脂肪和骨骼肌组织蛋白质含量分别为4±1和177±10µg Protein·mg tissue−1 （P< 0.05）。总而言之，人体脂肪每毫克组织中含有骨骼肌中28%的DNA， 9%的RNA和2%的蛋白质。这些信息应该在广泛的人类临床研究设计和各种实验室分析中有用。这项研究研究了年轻活跃个体的脂肪和骨骼肌组织的DNA、RNA和蛋白质含量。研究了一系列优化步骤，以确定提取高产量和高质量生物分子的最佳方法。这些发现导致了分子生物学分析对脂肪组织需求的知识缺口，由于对脂肪组织研究涉及人体运动生理学研究的兴趣日益浓厚，这一点越来越重要。
The purpose of this project was to provide a profile of DNA, RNA, and protein content in adipose tissue, which is relatively understudied in humans, to gain more insight into the amount of tissue that may be required for various analyses. Skeletal muscle tissue was also investigated to provide a direct comparison into potential differences between these two highly metabolically active tissues. Basal adipose and skeletal muscle tissue samples were obtained from 10 (7 M, 3 W) recreationally active participants [25 ± 1 yr; 84 ± 3 kg, maximal oxygen consumption (V̇o2max): 3.5 ± 0.2 L/min, body fat: 29 ± 2%]. DNA, RNA, and protein were extracted and subsequently analyzed for quantity and quality. DNA content of adipose and skeletal muscle tissue was 52 ± 14 and 189 ± 44 ng DNA·mg tissue−1, respectively (P< 0.05). RNA content of adipose and skeletal muscle tissue was 46 ± 14 and 537 ± 72 ng RNA·mg tissue−1, respectively (P< 0.05). Protein content of adipose and skeletal muscle tissue was 4 ± 1 and 177 ± 10 µg protein·mg tissue−1, respectively (P< 0.05). In summary, human adipose had 28% of the DNA, 9% of the RNA, and 2% of the protein found in skeletal muscle per mg of tissue. This information should be useful across a wide range of human clinical investigation designs and various laboratory analyses.NEW & NOTEWORTHYThis investigation studied DNA, RNA, and protein contents of adipose and skeletal muscle tissues from young active individuals. A series of optimization steps were investigated to aid in determining the optimal approach to extract high-yield and high-quality biomolecules. These findings contribute to the knowledge gap in adipose tissue requirements for molecular biology assays, which is of increasing importance due to the growing interest in adipose tissue research involving human exercise physiology research.