In vitro antibacterial activity of ZnO and Nd doped ZnO nanoparticles against ESBL producing Escherichia coli and Klebsiella pneumoniae.
In vitro antibacterial activity of ZnO and Nd doped ZnO nanoparticles against ESBL producing Escherichia coli and Klebsiella pneumoniae.
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DOI：
10.1038/srep24312
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发表时间：
2016-04-13
影响因子：
4.6
通讯作者：
Ravi G
中科院分区：
文献类型：
--
作者：
Hameed AS;Karthikeyan C;Ahamed AP;Thajuddin N;Alharbi NS;Alharbi SA;Ravi G
采用共沉淀法合成了纯 ZnO 和掺钕 (Nd) ZnO 纳米颗粒 (NPs)。合成的纳米颗粒保留了纤锌矿六方结构。 FESEM 研究表明，ZnO 和 Nd 掺杂 ZnO NPs 分别呈现纳米棒状和纳米花状形态。 FT-IR 光谱证实了 ZnO 和 Nd 掺杂 ZnO NP 的 Zn-O 伸缩带分别位于 422 和 451 cm−1 处。根据UV-VIS光谱测量，在各样品中在373nm和380nm附近发现了激子峰。光致发光测量表明，由于锌空位、氧空位和表面缺陷，宽发射由十个不同的带组成。针对产生超广谱β-内酰胺酶（ESBL）的大肠杆菌和肺炎克雷伯菌菌株进行的抗菌研究表明，Nd 掺杂的 ZnO NPs 比纯 ZnO NPs 具有更强的抗菌作用。通过共聚焦激光扫描显微镜（CLSM）分析，通过细菌的细胞收缩、细胞壁和细胞膜的解体以及死细胞证实了细胞的凋亡性质。 SEM分析显示，由于细胞膜完整性受损，细菌存在活力丧失，这与细胞壁的损伤高度一致。
Pure ZnO and Neodymium (Nd) doped ZnO nanoparticles (NPs) were synthesized by the co-precipitation method. The synthesized nanoparticles retained the wurtzite hexagonal structure. From FESEM studies, ZnO and Nd doped ZnO NPs showed nanorod and nanoflower like morphology respectively. The FT-IR spectra confirmed the Zn-O stretching bands at 422 and 451 cm−1 for ZnO and Nd doped ZnO NPs respectively. From the UV-VIS spectroscopic measurement, the excitonic peaks were found around 373 nm and 380 nm for the respective samples. The photoluminescence measurements revealed that the broad emission was composed of ten different bands due to zinc vacancies, oxygen vacancies and surface defects. The antibacterial studies performed against extended spectrum β-lactamases (ESBLs) producing strains of Escherichia coli and Klebsiella pneumoniae showed that the Nd doped ZnO NPs possessed a greater antibacterial effect than the pure ZnO NPs. From confocal laser scanning microscopic (CLSM) analysis, the apoptotic nature of the cells was confirmed by the cell shrinkage, disorganization of cell wall and cell membrane and dead cell of the bacteria. SEM analysis revealed the existence of bacterial loss of viability due to an impairment of cell membrane integrity, which was highly consistent with the damage of cell walls.