Dysregulated long non-coding RNAs in pleomorphic adenoma tissues of pleomorphic adenoma gene 1 transgenic mice
Dysregulated long non-coding RNAs in pleomorphic adenoma tissues of pleomorphic adenoma gene 1 transgenic mice
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DOI：
10.3892/mmr.2019.10149
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发表时间：
2019-04
影响因子：
3.4
通讯作者：
Wanlin Xu;Limin Liu;Hao Lu;J-W Fu;Chenping Zhang;Wenjun Yang;Shukun Shen
中科院分区：
文献类型：
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作者：
Wanlin Xu;Limin Liu;Hao Lu;J-W Fu;Chenping Zhang;Wenjun Yang;Shukun Shen
长链非编码RNA（lncRNA）已被证明在多种人类疾病中起重要作用。然而，他们参与发展多形性腺瘤（PA）在唾液腺尚未被检查。在本研究中，基因芯片分析的lncRNA和mRNA表达谱在多形性腺瘤基因1（PLAG 1）转基因小鼠进行。然后，利用生物信息学工具，包括基因本体论（GO）、京都基因和基因组百科全书（KEGG）途径富集和lncRNA-mRNA共表达网络分析，对PA相关差异表达基因进行预测。转基因小鼠和对照小鼠的比较表明，总共9，110个lncRNA和7，750个mRNA显著差异表达（倍数变化>2; P<0.05）。随后，随机选择6个lncRNA进行进一步分析，其中5个通过定量聚合酶链反应验证为PA中的差异表达，支持本研究中采用的方法。GO和KEGG富集分析的差异表达的mRNA显示，这些mRNA与参与PA的发展过程中的一些密切相关。此外，lncRNA-mRNA共表达网络表明，某些lncRNA可能通过与许多核心基因相互作用在PA的发病机制中发挥重要作用。总之，这些结果表明，与对照小鼠相比，从PLAG 1转基因小鼠获得的PA组织中的lncRNA和mRNA差异表达。这些差异表达的lncRNA可能作为PA的新的生物标志物和治疗靶点。
Long non-coding RNAs (lncRNAs) have been proven to serve vital roles in various human diseases. However, their involvement in the development of pleomorphic adenoma (PA) in the salivary gland has yet to be examined. In the present study, microarray analysis of the lncRNA and mRNA expression profiles in pleomorphic adenoma gene 1 (PLAG1) transgenic mice was performed. Next, bioinformatics tools were used to predict the differentially expressed genes associated with PA, including Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment and lncRNA-mRNA co-expression network analyses. Comparison of the transgenic and control mice demonstrated that a total of 9,110 lncRNAs and 7,750 mRNAs were significantly differentially expressed (fold change >2; P<0.05). Subsequently, six lncRNAs were randomly selected for further analysis, and five of these were validated as differentially expressed in PA by quantitative polymerase chain reaction, supporting the methodology employed in the current study. The GO and KEGG enrichment analysis of the differentially expressed mRNAs revealed that these mRNAs were closely associated with a number of processes involved in the development of PA. Furthermore, the lncRNA-mRNA co-expression network indicated that certain lncRNAs may serve vital roles in the pathogenesis of PA by interacting with a number of core genes. Taken together, these results indicated that lncRNAs and mRNAs were differentially expressed in PA tissues obtained from PLAG1 transgenic mice as compared with those from control mice. These differentially expressed lncRNAs may act as novel biomarkers and therapeutic targets for PA.