ANION-SENSITIVE ATPASE IN RABBIT CORNEAL ENDOTHELIUM AND ITS RELATION TO CORNEAL HYDRATION
ANION-SENSITIVE ATPASE IN RABBIT CORNEAL ENDOTHELIUM AND ITS RELATION TO CORNEAL HYDRATION
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DOI：
10.1016/0014-4835(77)90177-4
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发表时间：
1977-01-01
影响因子：
3.4
通讯作者：
RILEY, MV
中科院分区：
文献类型：
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作者：
RILEY, MV
兔角膜内皮中镁离子激活的ATPase活性依赖于介质的阴离子组成，哇巴因不抑制该酶的活性。在存在**图形**的情况下，表现出最大的阴离子依赖的镁ATPase活性。然后是醋酸盐、氯离子、.**图形**。SCN-和OCN-。在存在镁离子、镁离子时，ATP的最大水解率。**图表**。Mg~(2+)+Na~++K~+分别为5.61。0.7，0.93.+-.0.8和6.74。+-。0.7µm ol hr-1 mg-1蛋白。每种ATPase活性的主要部分是在粗线粒体内发现的。在**图形**在场的情况下进行的活动当添加50 mM SCN或10 mM OCN时，抑制率为65%。当将这些浓度的抑制剂添加到灌流完整、隔离的角膜的介质中时，会导致它们将液体输送出组织的能力恶化。阴离子敏感的ATPase显然在内皮的能量代谢中起着重要的定量作用，并被认为是活跃的。**图表**。通过细胞层的运输可能受到这种酶的影响。
The activity of Mg2+-activated ATPase in the corneal endothelium of the rabbit is dependent on the anionic composition of the medium and is not inhibited by ouabain. Maximum anion-dependent Mg2+ ATPase activity was demonstrated in the presence of .**GRAPHIC**. followed by acetate, Cl-, .**GRAPHIC**. SCN- and OCN-. The maximum rates of ATP hydrolyzed in the presence of Mg2+, Mg2+ + .**GRAPHIC**. and Mg2+ + Na+ + K+ were 5.61 .+-. 0.7, 0.93 .+-. 0.8 and 6.74 .+-. 0.7 .mu.mol hr-1 mg-1 protein, respectively. The major part of each of these ATPase activities was found in a crude mitochondrial fraction. Activity in the presence of .**GRAPHIC**. was inhibited by 65% when 50 mM SCN- or 10 mM OCN- was added to the medium. These concentrations of inhibitor, when added to a medium perfusing intact, isolated corneas, caused a deterioration of their ability to transport fluid out of the tissue. The anion-sensitive ATPase apparently plays an important quantitative role in the energy metabolism of the endothelium, and the postulated active .**GRAPHIC**. transport across the cell layer may be influenced by this enzyme.