Forensic tracing of horse identities using urine samples and DNA markers
Forensic tracing of horse identities using urine samples and DNA markers
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使用尿液样本和 DNA 标记对马身份进行法医追踪
DOI：
10.1080/10495399609525855
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发表时间：
1996
影响因子：
3.7
通讯作者：
L. Andersson
中科院分区：
文献类型：
--
作者：
S. Marklund;K. Sandberg;L. Andersson
摘要：采用9个微卫星标记和1个线粒体D - loop单链构象多态性（SSCP）对马尿样进行DNA检测的可能性进行了测试。用简单的方法从不同尿量（每次反应5、40和80 μ1尿）中制备用于聚合酶链反应（PCR）分析的基因组DNA。从尿液和血液样本中获得一致的遗传数据，除了在少数情况下，每个制备的最小尿量的PCR分析只显示杂合子中的一个等位基因。在这些情况下，一系列反应表明这两个等位基因是随机扩增的，可能是因为初始目标分子数量非常少。在大多数情况下，PCR产物的数量随着每次制备的体积的增加而增加，但三个样品显示PCR产物的急剧减少。对于这些样品，最浓缩的制备物的超滤显着改善了结果，并使基因分型成为可能。
Abstract The possibility to carry out DNA testing using horse urine samples collected for doping control was tested using nine microsatellite markers and one mitochondrial D‐loop single strand conformation polymorphism (SSCP). Genomic DNA for polymerase chain reaction (PCR) analysis was prepared from different volumes of urine (5, 40 and 80 μ1 urine per reaction) using a simple protocol. Consistent genetic data were obtained from urine and blood samples except in a few cases where PCR analysis of the smallest urine volume per preparation only revealed one allele in heterozygotes. In these cases serial reactions showed that the two alleles were randomly amplified, probably because of a very low number of initial target molecules. In most cases the amount of PCR product increased with increasing volume per preparation but three samples showed a drastic reduction of PCR product. For these samples ultrafiltration of the most concentrated preparation dramatically improved the results and enabled genotyping wit...