Tissue engineering of arteries by directed remodeling of intact arterial segments
Tissue engineering of arteries by directed remodeling of intact arterial segments
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DOI：
10.1089/107632703322066642
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发表时间：
2003-06-01
影响因子：
--
通讯作者：
Gooch, KJ
中科院分区：
文献类型：
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作者：
Clerin, V;Nichol, JW;Gooch, KJ
传统的体外构建组织工程化动脉的方法依赖于培养细胞的扩增以接种合适的支架。在大多数设想的应用中，小的自体血管将被收获并用作这些细胞的来源。我们认为，小的自体动脉，而不是来自它们的细胞，可能是工程动脉的一个有吸引力的起点。这种方法利用了完整动脉响应其机械环境中的慢性变化而生长和重塑的能力。在离体灌注系统中纵向拉伸幼年猪（类似于30-kg）的颈动脉9天。这导致生理纵向应力下动脉长度增加40%，无应力时增加20 +/- 3%。对照动脉以其生理负载长度离体灌注9天。与新鲜采集的动脉相比，对照和伸长的动脉显示出天然外观（肉眼和组织学）、优异的活力（细胞构成和线粒体活性）、正常的血管活性和相似的机械性能（极限应力和极限应变）。增长，而不是现有质量的重新分配，有助于延长，如动脉重量增加所证明的。还介绍和讨论了新生猪和青少年猪动脉伸长的结果。
Traditional approaches to generating tissue-engineered arteries in vitro rely on expansion of cells in culture to seed appropriate scaffolds. In most envisioned applications, small autologous blood vessels would be harvested and used as a source for these cells. We propose that small autologous arteries, not the cells derived from them, may be an attractive starting point for engineered arteries. This approach capitalizes on the ability of intact arteries to grow and remodel in response to chronic changes in their mechanical environment. Carotid arteries from juvenile (similar to30-kg) pigs were stretched longitudinally in an ex vivo perfusion system over 9 days. This resulted in a 40% increase in artery length at physiological longitudinal stress and a 20 +/- 3% increase when unstressed. Control arteries were perfused for 9 days ex vivo at their physiological loaded length. Control and elongated arteries displayed native appearance (macroscopic and histological), excellent viability (cellularity and mitochondrial activity), normal vasoactivity, and similar mechanical properties (ultimate stress and ultimate strain) as compared with freshly harvested arteries. Growth, as opposed to just redistribution of existing mass, contributed to elongation as evidenced by an increase in artery weight. Results on elongation of arteries from neonatal and adolescent pigs are also presented and discussed.