Streptokinase and urokinase activation of human, chimpanzee and baboon plasminogen.
Streptokinase and urokinase activation of human, chimpanzee and baboon plasminogen.
复制标题
人类、黑猩猩和狒狒纤溶酶原的链激酶和尿激酶激活。
DOI：
10.1055/s-0038-1653704
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发表时间：
1971
期刊：
影响因子：
--
通讯作者：
Hampton Jw
中科院分区：
文献类型：
--
作者：
McKee Pa;Lemmon Wb;Hampton Jw
人、黑猩猩和狒狒的纤溶酶原依次被发现对链激酶（SK）激活的敏感性逐渐降低。当使用种属特异性纤维蛋白或酪蛋白作为底物时，并且当改变以下条件时，三种灵长类动物纤溶酶原的相对活化保持相同：1. SK浓度; 2.纤溶酶原浓度;和3. SK-纤溶酶原-底物反应混合物的孵育时间。在相同的条件下，三种灵长类纤溶酶原很容易被人尿激酶（UK）激活，并没有一致的顺序激活UK观察。我们的研究结果表明，纤溶酶原与SK相互作用的网站在进化过程中发生了变化，而英国激活三个纤溶酶原的网站变化不大。灵长类动物纤溶酶原对SK激活的易感性是否是一种特别选择的遗传性状，或者仅仅是由于其他原因而发生的纤溶酶原分子结构变化的实验室指标，仍然不清楚。
Human, chimpanzee and baboon plasminogens, in that order, were found to be decreasingly susceptible to streptokinase (SK) activation. The relative activation of the three primate plasminogens remained the same when species specific fibrin or casein was used as substrate and when the following conditions were varied: 1. SK concentration; 2. plasminogen concentration; and 3. length of incubation of the SK-plasminogen-substrate reaction mixture. Under the same conditions, the three primate plasminogens were readily activated by human urokinase (UK) and no consistent order of activation by UK was observed. Our findings suggest that the site on plasminogen which interacts with SK has undergone changes during evolution whereas the site at which UK activates each of the three plasminogens has changed little. It remains obscure whether the susceptibility of primate plasminogen to SK activation is a specifically selected genetic trait or is simply a laboratory indication of structural changes in the plasminogen molecule that have occurred for other reasons.