Morphometric Analysis of Aqueous Humor Outflow Structures with Spectral-Domain Optical Coherence Tomography
Morphometric Analysis of Aqueous Humor Outflow Structures with Spectral-Domain Optical Coherence Tomography
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DOI：
10.1167/iovs.11-9229
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发表时间：
2012-08-01
影响因子：
4.4
通讯作者：
Schuman, Joel S.
中科院分区：
文献类型：
--
作者：
Francis, Andrew W.;Kagemann, Larry;Schuman, Joel S.
目的。描述在尸体眼睛活动 AH 流出期间用 360 度虚拟铸件可视化的人房水 (AH) 流出微脉管系统的形态细节，并将这些结构与腐蚀铸件研究进行比较。方法。在主动 AH 流出过程中，使用谱域光学相干断层扫描 (SD-OCT) 和宽带超发光二极管阵列对供体眼睛 (n = 7) 和体内眼睛 (n = 3) 的传统 AH 流出路径进行成像。调整数字图像对比度以隔离 AH 微脉管系统，并在 3D 查看器中查看图像。另外的眼睛 (n = 3) 用含有荧光示踪剂微球的模拟 AH 进行灌注，以比较微脉管系统模式。 结果。观察揭示了从施累姆氏管（SC）到浅表巩膜内静脉丛（ISVP）的传统流出路径的组成部分。我们的研究和腐蚀铸件中的表面 ISVP 均由相互连接的小静脉 (10-50 μm) 组成，形成六边形网状结构。较大的径向拱廊（50-100μm）排出了最接近SC的区域，并与较大的曲折血管（>100μm）会聚。 360 度虚拟铸造非常接近腐蚀铸造研究。示踪剂研究证实了我们的发现。在原始和对比度增强的荧光图像中，示踪剂装饰了从角膜缘向后延伸的几个较大的血管（50-100μm）。可以看到较小的示踪剂标记血管（30-40μm）在较大血管之间分支，并表现出类似的六边形网络图案。结论。 SD-OCT 能够对体内或体外的传统流出途径进行详细的形态分析，其细节可与腐蚀铸造技术相媲美。 （投资眼科可见科学；53：5198-5207）DOI：10.1167/iovs.11-9229
PURPOSE. To describe morphometric details of the human aqueous humor (AH) outflow microvasculature visualized with 360-degree virtual castings during active AH outflow in cadaver eyes and to compare these structures with corrosion casting studies.METHODS. The conventional AH outflow pathways of donor eyes (n = 7) and eyes in vivo (n = 3) were imaged with spectral-domain optical coherence tomography (SD-OCT) and wide-bandwidth superluminescent diode array during active AH outflow. Digital image contrast was adjusted to isolate AH microvasculature, and images were viewed in a 3D viewer. Additional eyes (n = 3) were perfused with mock AH containing fluorescent tracer microspheres to compare microvasculature patterns.RESULTS. Observations revealed components of the conventional outflow pathway from Schlemm's canal (SC) to the superficial intrascleral venous plexus (ISVP). The superficial ISVP in both our study and corrosion casts were composed of interconnected venules (10-50 mu m) forming a hexagonal meshwork. Larger radial arcades (50-100 mu m) drained the region nearest SC and converged with larger tortuous vessels (>100 mu m). A 360-degree virtual casting closely approximated corrosion casting studies. Tracer studies corroborated our findings. Tracer decorated several larger vessels (50-100 mu m) extending posteriorly from the limbus in both raw and contrast-enhanced fluorescence images. Smaller tracer-labeled vessels (30-40 mu m) were seen branching between larger vessels and exhibited a similar hexagonal network pattern.CONCLUSIONS. SD-OCT is capable of detailed morphometric analysis of the conventional outflow pathway in vivo or ex vivo with details comparable to corrosion casting techniques. (Invest Ophthalmol Vis Sci.;53:5198-5207) DOI:10.1167/iovs.11-9229