Overexpression of the wild potato eIF4E-1 variant Eva1 elicits Potato virus Y resistance in plants silenced for native eIF4E-1
Overexpression of the wild potato eIF4E-1 variant Eva1 elicits Potato virus Y resistance in plants silenced for native eIF4E-1
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DOI：
10.1007/s11248-011-9576-9
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发表时间：
2012-10-01
影响因子：
3
通讯作者：
Rommens, Caius M.
中科院分区：
文献类型：
--
作者：
Duan, Hui;Richael, Craig;Rommens, Caius M.
马铃薯Y病毒（Potato virus Y， PVY）是马铃薯栽培（Solanum tuberosum）最重要的商业病原菌，在世界范围内造成薯类品质和产量的严重损失。特定的野生马铃薯品种表现出对PVY的抗性，但将该性状转移到栽培材料的努力尚未产生广泛采用的品种。由于宿主因子eIF4E-1特定区域的氨基酸替换通常赋予对各种作物的抗性，我们对野生马铃薯植物中表达的相关基因进行了测序。一种新的eIF4E-1变异，在这里被命名为Eva1，在沙棘棘、半棘棘和棘棘中被鉴定出来。与栽培马铃薯（S. tuberosum）同源物相比，该蛋白含有十个不同位置的氨基酸取代。在酵母双杂交系统中，Eva1不能结合VPg， VPg是一种具有感染性的病毒蛋白。相关cDNA的过表达使PVY对原生eIF4E-1基因沉默的转基因马铃薯具有抗性。由于Eva1基因源与马铃薯有性亲和性，所描述的分子策略可用于培育“基因内”马铃薯品种。
Potato virus Y (PVY) is the most important viral pathogen of cultivated potato (Solanum tuberosum) from a commercial perspective, causing severe losses in both tuber quality and yield worldwide. Specific accessions of wild potato species exhibit resistance against PVY but efforts to transfer the trait to cultivated material have not yielded widely adopted varieties. Because amino acid substitutions at specific domains of host factor eIF4E-1 often confer resistance to various crops, we sequenced the associated genes expressed in wild potato plants. A novel eIF4E-1 variant, designated here as Eva1, was identified in S. chacoense, S. demissum, and S. etuberosum. The protein contains amino acid substitutions at ten different positions when compared to its cultivated potato (S. tuberosum) homolog. In the yeast two-hybrid system, Eva1 failed to bind VPg, a viral protein required for infectivity. Overexpression of the associated cDNA conferred PVY resistance to transgenic potato plants silenced for the native eIF4E-1 gene. Because the gene sources of Eva1 are sexually compatible with potato, the molecular strategies described can be employed to develop 'intragenic' potato cultivars.